Molecular diagnosis of Avian Influenza Virus from different Avian species
Molecular diagnosis of Avian Influenza Virus from different Avian species
Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2
ABSTRACT
Background: The AIV is one of the most important poultry viral infections that represent one of the greatest concerns for public health. AIV classified within Orthomyxoviridae that is highly mutating virus. AIV was founded in Egypt since 2006. Control and prevention of AIV done via good managemental procedures, as well as strict quarantine measures and enhanced bio-security. Regular diagnosis and viral screening will be helpful in the eradication of the new strains to predict the newly coming waves of infection and to be able to develop the recommended vaccines. Objective: Our study aimed for Molecular Diagnosis of Avian influenza virus (AIV) from Different Avian Species that would be useful to detect the current strains present in Egypt to use it in vaccination program. Methods:In this study, we collected 122 sample representing 12 different area in Egypt and seven different species of poultry produced and traded in Egypt. These samples were isolated on SPF eggs, detected for AIV infection Via HA, RT-PCR and gene sequencing. We analyzed the sequence on gene bank to compare it with the other AIV strains
Results: We found 35 and 55 samples showed positive result for H5 and H9 respectively. After sequencing and comparing with the other AIV strains we found that the isolated strains showed close similarity with the AIV strains that were present in Egypt from 2006 to 2008 (subclade 2) and showed low similarity to the strains that were present in Egypt from 2008 to 2015.
Conclusion: AIV H5 strain that isolated from Chicken, Duck and pigeon, and AIV H9 strain that isolated from chicken show where mutated strains from previously AIV circulating in Egypt from 2006 to 2008. Therefore, we recommend incorporation of these such isolates in any newly prepared AIV vaccines and vaccination program.
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July 2019
Vol. 6, Pages 1-101
