Antioxidant Role of Nano-Piperine and Ascorbic Acid in Some Blood Parameters and Liver in Dexamethasone Treated Male Rats
Antioxidant Role of Nano-Piperine and Ascorbic Acid in Some Blood Parameters and Liver in Dexamethasone Treated Male Rats
Saadeya Ali Lefelef Al-Gnami1, Fatima Ibrahim Mohammad2, Huda Jassim Mohammed Al-Helali3
ABSTRACT
Glucocorticoids are extensively utilized in veterinary medicine because to their anti-inflammatory and immunosuppressive properties. Vitamin C and Nanopiperine are crucial in mitigating the effects of free radicals by acting as antioxidants.The study investigates the positive impacts of piperine nanoparticles and vitamin C on several blood parameters and liver tissue in male rats treated with dexamethasone. The forty-eight male rats were randomly divided into six groups. Nanopiperine (15 mg/Kg.B.W) was given orally to the T1 group. Vitamin C (25 mg/Kg.B.W) was given orally to the T2 group. The T3 group received orally dexamethasone (2 mg/Kg.B.W). Nanopiperine and dexamethasone were given orally to the T4 group at the same dose. The T5 group received a single oral dosage of vitamin C and dexamethasone. After the end of experiment the animals were dissected and blood samples were collected for measuring Hb levels, PCV, mean cell volumes, MCHC, and RBCs, and the livers samples were removed from all animals and reserved them in formalin 10% for histological examination. The results of the present study exhibited a notable rise (p<0.05) in RBC count, Hb, PCV, MCV, MCH, and MCHC in T1 and T2 as compared with control group. While these parameters were significantly decreased (p<0.05) in T3 group that drenched with dexamethasone. T4 and T5 exposed a substantial rise(p<0.05) in all these blood parameters when compared to T3. Exa-mination of liver tissue in control , T1 and T2 exposed normal tissue appearance. T3 exposed the normal radial arrangements of hepatocyte with a trifling infiltration of inflammatory cells and multiplying in kuffer cells. T4 and T5 revealed normal appear of hepatocyte with a clear central nucleus site. This research can be concluded that nano-piprine and Vit. C have a great role in reducing the side effect of dexamethasone on liver and red blood cells.The results related to the piperine nanoparticles was better than vitamin C.
Keywords | Nanopiperine, Ascorbic acid, Dexamethasone, Oxidative stress, antioxidant, Liver, Rats
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September 2024
Vol. 12, Iss. 9, pp. 1622-1845
