Role of SOD1 Gene (�251A/G) Polymorphism in Changing the level of Serum Metals and Minerals in Rheumatoid Arthritis Patients
Role of SOD1 Gene (�251A/G) Polymorphism in Changing the level of Serum Metals and Minerals in Rheumatoid Arthritis Patients
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
ABSTRACT
Oxidative stress is culprit of several pathological and physiological conditions. One of them is Rheumatoid arthritis (RA). The exact cause of RA is still unknown. Reduced activity of antioxidants enzymes because of polymorphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A total of 400 individuals including age and gender matched healthy individuals (control group) and RA patients were genotyped. Detection of rs2070424 polymorphism was carried out using allele specific PCR based amplification strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant from HWE in RA group. The results of present study indicates that Pb and Cr concentration differ significantly between AA, AG and GG genotypes of SOD1 (rs2070424) gene polymorphism in RA patients as compared to control group (p<0.05). The present finding indicates that SOD1 (rs2070424) gene polymorphism is not associated with pathogenesis of RA; however levels of Pb and Cr may impart strong influence towards the development of RA in the presence of this polymorphism.
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