Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle
Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
ABSTRACT
The aim of this research was to evaluate the quality characteristics of X- and Y-chromosome-bearing sperms separated by using albumin gradient and swim-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53.00±7.93% and 54.08±8.76%, respectively. In swim-up method the average motility, PMI and AI of sperms bearing X- and Y-chromosome was recorded 60.83±7.54% and 70.00±8.94%, 54.85±7.40% and 58.43±8.08%, and 53.37±8.76% and 58.96±8.41%, respectively. The percentage of sperm motility, PMI and AI was higher (P < 0.05) in swim-up than in the albumin gradient technique. From the results, it could be concluded that the motility, PMI and AI was slightly higher for Y-sperm than X-sperm regardless of semen sexing technique.
Keywords | Pesisir bull, Albumin gradient, Swim up, Motility, PMI, AI
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