Molecular detection of DNA component 6 (DNA-N) of banana bunchy top virus isolated from Egypt
Molecular detection of DNA component 6 (DNA-N) of banana bunchy top virus isolated from Egypt
Mansour, L. L.*; Othman, B. A. **; Abd-EL Ghaffar, M. H. **; Eman, M. Marai** and Sahar, A. Youssef*
ABSTRACT
Banana bunchy top virus (BBTV) is considered the most serious disease
affecting banana in Egypt.BBTV was isolated from infected banana(Musa
acuminata cv Grandnain)obtained from EL-Behira, Egypt
byPentalonianigronervosa .Polymerase chain reaction (PCR) was used to detect
BBTV in infected plants and banana aphid (P.nigronervosa) using specific primer
of DNA-1 for BBTV. The results showed that amplified PCR product with the
expected size 476 bp for both infected banana and aphid. PCR was used to detect
component 6 of BBTV-DNA using specific primer at expected size 813 bp.The
isolated component was cloned into PCR™-4-TOPO vector (3.956~kb) and were
transformed using minipreperation method. Component-6 was sequenced to
determine the phylogenetic with other published isolates. Phylogenetic tree showed
the identity percentage of Egyptian isolate group of BBTV-component- 6 with
component 6 of Indian, Taiwanese and Pakistani isolates group was 97%, whereas
it was 84% in case ofChinese isolate group.
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December 2013
Vol. 10, Pages 1-171
