Submit or Track your Manuscript LOG-IN

Genome Characterization of Probiotic Lactobacillus delbrueckii Subsp. bulgaricus Strain NDO2 Isolated from Traditional Yogurt using High Throughput Next Generation Sequencing

Genome Characterization of Probiotic Lactobacillus delbrueckii Subsp. bulgaricus Strain NDO2 Isolated from Traditional Yogurt using High Throughput Next Generation Sequencing

Mati Ullah1*, Muhammad Rizwan2, Ali Raza3, Xianlin Zhao4, Yanling Sun4, Sarah Gul5, Muhammad Ihtesham Waheed6, Muhammad Nadeem Khan7, Alvina Gul8, Sami Ullah Jan9* and Chao Huang4*

1Department of Microbiology, Faculty of Veterinary & Animal Sciences, The University of Agriculture, Dera Ismail Khan, Pakistan
2College of Animal Sciences and Technology, Anhui Agricultural University, Hefei, Anhui, China
3Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
4Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, China
5Department of Biological Sciences, Faculty of Basic and Applied Sciences, International Islamic University, Islamabad, Pakistan
6Department of Plant Breeding and Genetics, University of Agriculture, Peshawar
7Department of Microbiology, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, Pakistan
8Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, H-12 Campus, Islamabad, Pakistan
9Department of Bioinformatics and Biosciences, Faculty of Health & Life Sciences, Capital University of Science and Technology, Islamabad, Pakistan
 
*      Corresponding author: samiullahjan@gmail.com, 67731020@qq.com; matiqau@gmail.com

ABSTRACT

Probiotic bacteria present in fermented milk and its products have a long history of safety and health associated uses. If bacteria isolated from yogurt is characterized technologically and gnomically, it could be used in fermentation for selective purposes. In the current study, we have comprehensively analyzed the genome of Lactobacillus delbrueckii subsp. bulgaricus NDO2 isolated from traditional yogurt samples using the next-generation sequencing approach. The GC contents of the selected genome were 45.3%. Among the total 2284 genes identified, 970 genes similar in three genomes make the core genome portion. The subsystems, category distribution analysis revealed “carbohydrates” was the largest subsystem, followed by “protein metabolism”. The eggNOG functional analysis revealed that the COGs associated with “Carbohydrate transport and metabolism were dominant followed by those associated with “Replication, recombination, and repair” while the biological pathway analysis by the Kyoto encyclopedia of genes and genomes (KEGG) database identified the metabolic and biosynthesis of secondary metabolites pathway as the dominant one. These findings provide a broader genomic understanding of the famous probiotics bacterial species for general as well as commercial applications. This study could also be used basis for similar studies.

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe