Pseudomonas Species Isolated from Camels: Phenotypic, Genotypic and Antimicrobial Profile
Pseudomonas Species Isolated from Camels: Phenotypic, Genotypic and Antimicrobial Profile
Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3*
ABSTRACT
One of the important livestock economies are camels which adapt with adverse environmental conditions and provide milk, meat, wool, hides, and skin. Gram-negative Pseudomonas aeruginosa is harbor multidrug antimicrobial resistance of camel has serious consequences for human health, so, this study aimed to characterized of P. aeruginosa especially extended spectrum β-lactamases (ESBL) producing one; phenotypic and genotypic. The isolates of P. aeruginosa were confirmed biochemically by a Vitek 2 compact system (bioMe´rieux). Suspected P. aeruginosa colonies carry ESBL were 56.6% by the double disc synergy test (DDST). Detection of virulence genes using pslA, toxA and exoU genes revealed that 29.4%, 23.5% and 17.6% respectively. Molecular detection of ESBLs encoding genes in P. aeruginosa recorded that blaTEM genes blaSHV and blaCTXM genes were detected in percentages of 64.7%, 47.0 % and 29.4%, respectively. Finally, ESBL P. aeruginosa showing multidrug antimicrobial resistance that detected by mexR gene.
Keywords | Camels, P. aeruginosa, ESBL, Antibiotic resistance, Virulence genes
To share on other social networks, click on any share button. What are these?
