Biological Activity and Pharmaco-Therapeutic Efficiency of Calligonum leucocladum B. Dosage Forms in the Treatment of Endometritis of Cows
Biological Activity and Pharmaco-Therapeutic Efficiency of Calligonum leucocladum B. Dosage Forms in the Treatment of Endometritis of Cows
Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova*
ABSTRACT
The aim of this study is to obtain dosage forms from plant raw materials Calligonum leucocladum, to study their pharmacotherapeutic activity and use for the treatment of endometritis in cows. A total of 270 cows were examined by clinical methods after calving, of which 45 cows showed signs of acute endometritis. Subsequently, the bacterial background of uterine mucus in sick cows was investigated and St. pyogenes, E. faecalis, and E. coli were isolated. For the complex treatment of acute postpartum endometritis, the cows of the experimental groups were treated with the phytopreparation C. leucocladum. For comparison, the drug «Biometrosanit» was used in the treatment of cows of the control group. Phytopreparations of C. leucocladum were suppositories obtained from extracts for intrauterine administration, with 20% and 40% content of C. leucocladum. Previously, phytopreparations were tested for antimicrobial (diffusion in agar by serial double dilutions) and antiradical activity (comparison with Butylhydroxyanisole (BHA), as well as for toxicity and the ability to cause irritation or an allergic reaction from the mucous membranes. In cows that used a suppository with a 40% content of C. leucocladum, the earliest recovery periods were revealed (6 ± 0.5 days), the number of cured animals was 80%. The use of intrauterine suppositories with 20% content of C. leucocladum did not provide a high therapeutic effect (53.3%). The cows of the experimental groups showed positive dynamics in the content of metabolic components in the blood. The compared drug «Biometrosanit» caused the recovery of 73.3% of control animals. Medicinal forms of C. leucocladum were obtained by drying, grinding, extracting extract from roots, stems in the Soxlet extractor. The pharmacological and therapeutic efficacy of the ethanol extract of C. leucocladum has been studied. A toxicity test was carried out on white mice using 10%, 20%, 40% concentration of C. leucocladum, no death of mice was recorded within 30 days. When studying the irritating and allergic effects of C. leucocladum on rabbits, it was found that no irritating effect and allergic reaction was observed within 24 hours in animals with 10%, 20%, 40% content of C. leucocladum. Studies of the antimicrobial properties of C. leucocladum extract with 10%, 20%, 40% concentrations, suppositories containing 20% and 40% of C. leucocladum showed antimicrobial activity against E. coli, St. aureus. C. leucocladum shows the highest antimicrobial activity at 40% concentration of 18±0.1. C. leucocladum has a high antiradical activity. The study of the therapeutic efficacy of suppositories containing 20% and 40% of C. leucocladum in the treatment of endometritis in cows (n=45) provides recovery of 80% of cows. Thus, tests for biological activity, as well as the effect of C. leucocladum on mucous membranes, indicate its good antiradical properties and activity against E. coli, the absence of toxicity, irritating and allergenic properties. The information obtained by us will expand knowledge in the field of veterinary phytopharmacology and help develop safe and effective measures for the treatment of postpartum diseases of cows.
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