Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1
ABSTRACT
Thioacetamide (TAA) has been widely utilized in the development of animal models of hepatic damage by a number
of researchers. The current investigation was designed to evaluate changes in tissue and serum total proteins and
albumin along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution. Rats of 12h and 24h group were killed after 12 and 24h of TAA administration
respectively. Blood was obtained; livers were excised and processed for protein profile and transaminases activity
analysis. TAA injection resulted in significant alterations in serum proteins and transaminases levels. Significant
hypoproteinemia and hypoalbuminemia were observed in sera as well as liver tissue of both experimental groups
compared to control. Furthermore, transaminases activity was significantly increased for both; AST (P=0.0045) and
ALT (P=0.0015) in both experimental groups when compared to control. Taking in account these results it can be
concluded that TAA induces changes in the liver marker enzymes along with the change in the sera and tissue total
protein contents specifically albumin.
To share on other social networks, click on any share button. What are these?
