Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz
ABSTRACT
Enterotoxaemia, caused by Clostridium perfringens Type D, is a disease of domestic animals particularly sheep and goat widespread in Pakistan due to endemic outbreak in every spring season; therefore the current study was conducted for isolation and molecular identification of new strains of C. perfringens Type D for effective diagnosis, treatment and vaccination. A total of 100 fecal samples were collected aseptically from four different zones of district Peshawar during the period of February, 2019 to April, 2019. C. perfringens Type D was confirmed through bacterial culturing, Gram staining, biochemical tests and polymerase chain reaction (PCR) . Results revealed that 25 feacal samples collected from suspected goats were positive for C. perfringens Type D and the overall prevalence of enterotoxaemia in goats in district Peshawar was 25%. The prevalence of enterotoxaemia was 24%, 44%, 20% and 12% in zone I, II, III and IV respectively. Zone II showed the highest prevalence rate of 44%. However, the colony morphology, microscopy, biochemical tests and polymerase chain reaction of C. perfringens type D showed that PCR is an effective diagnostic and confirmatory tool for the toxinogenic typing of C. perfringens type D infection.
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