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Effect of Different Salinity Levels on Expression of Three Plasma Membrane Associated Genes of Penaeus monodon

Effect of Different Salinity Levels on Expression of Three Plasma Membrane Associated Genes of Penaeus monodon

Song Jiang, Dong-liang Liu, Fa-lin Zhou, Xian-bin Mo, Qi-bin Yang, Jian-hua Huang, Li-shi Yang and Shi-gui Jiang*

Key Lab. of South China Sea Fishery Resources Exploitation and Utilization, The Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China 510300;
Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, P.R. China

*      Corresponding author: jiangsg@21cn.com

ABSTRACT

In this study, three genes, PmATP synthase, PmNa+-K+-ATPase and PmToll receptor gene, were selected to study their relative expression in different tissues of Penaeus monodon. The results showed that the mRNA level of PmATP synthase gene was not significantly up-regulated under low salt stimulation (P> 0.05),while under high salinity stimulation, the transcription level of PmATP synthase mRNA reached the maximum at 16th h (P<0.05).The expression level of PmNa+-K+-ATPase mRNA increased at 4th h and 16th h, which was significantly higher than that in the beginning under low salt stimulation (P<0.05) and then decreased at 32nd h, ,while under high salinity stimulation, the expression level of PmNa+-K+-ATPase mRNA reached the highest at 8th h and 32nd h. The expression level of PmToll receptor mRNA reached the highest at 4th h after low salinity stimulation (P<0.05), and then down-regulated after 16th h, while under high salinity stress, the expression level was up-regulated and reached the maximum at 8th h, which was 2.4 times of that the in the control group (P<0.05) and decreased at 16th h after stress. PmATP synthase gene was relatively high expression, while PmNa+-K+-ATPase and PmToll receptor gene expression were low, so we guess that the large amounts of ATP of the body synthesis mainly use for the growth of the body. PmNa+-K+-ATPase and PmToll receptor gene expression are relatively high, which indicate that the body osmotic pressure regulation and the body’s immune process consume a lot of energy.
 

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Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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