Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest http://dx.doi.org/10.17582/journal.aavs/2023/11.1.150.158
Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest http://dx.doi.org/10.17582/journal.aavs/2023/11.1.150.158
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
ABSTRACT
The objective of this study was to determines the effect of guava fruit filtrate (GF) supplementation in Tris and Citrate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached at least 40%. The results showed that progressive motility and viability on the fourth day storage in extender Citrate-egg yolk (CEY) with 10% egg yolk and 10% GF were 50.4 ± 1.6% and 57.1 ± 1.6%, significantly higher (P<0.05) than control, TEY with 10% GF and Citrate with 10% egg yolk. The spermatozoa abnormality was 12.9 ± 0.3%, the lowest in extender Citrate with 10% egg yolk and 10% GF on the fourth-day storage. In conclusion, supplementation of GF to extender improves the spermatozoa quality of Brangus bull after sex activity break for a long time. Moreover, the best quality of spermatozoa is found in supplementing 10% GF to Citrate with 10% egg yolk extender.
Keywords | Brangus bull, Chilled storage, Extender, Guava, Semen
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