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Changes in Glutathione S-transferase Activity and Total Protein Contents of Labeo rohita

Changes in Glutathione S-transferase Activity and Total Protein Contents of Labeo rohita

Qaisra Siddique1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3


1Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan 
2Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
3Animal Sciences and Technology, Guangxi University, China

Corresponding author: Huma Naz,,


Abstract | This work was performed to evaluate glutathione S-transferase (GST) activityand total protein contents (TPCs) in tissues viz. brain, gills, kidney, heart, muscle and liver of Labeo rohita kept under sub-lethal dose (4.13 µgL-1) of chlorpyrifos. Fish was kept under chlorpyrifos stress for two months and samples were collected on weekly basis. It was noted that GST level varied significantly with duration. The GST level was raised in first 28 days after that it was dropped off up to 56-day. The trend of GST in fish tissues was observed as muscle<heart<brain<kidney<gills< liver. However, TPCs in selected tissues of stressed fishwas lower significantly as compared to control. 
Novelty Statement | Increased use of pesticides is not only hazardous to target animals but also to non-target aquatic organisms like fish. Pesticides can alter the biochemical parameters such as glutathione S-transferase in fish. The evaluation of GST is a useful bio-marker for assessing the environmental stress due to these pesticides.

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Punjab University Journal of Zoology


Vol.38, Iss. 1, Pages 01-135


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