Impact of Fig-Enriched Tris-Albumin Extender on the Quality of Boer Buck Spermatozoa
Impact of Fig-Enriched Tris-Albumin Extender on the Quality of Boer Buck Spermatozoa
Lalu Ahmad Zaenuri*, Rodiah Rodiah
ABSTRACT
This study aims to evaluate the effect of additional fig fruit filtrate in a Tris-albumin-based extender on the motility, viability, and morphology of Boer buck spermatozoa. The semen used in this study was collected from a 3-year-old Boer buck weighing 85 kg, which was well-trained, fertile, and free of both internal and external parasites. Ten semen samples were collected using an artificial vagina every four days over a period of ten consecutive days. The collected semen was divided into four tubes based on to the treatment extenders: FF0 (0 ml), FF1 (4 ml), FF2 (6 ml), and FF3 (8 ml) of fig fruit filtrate, each added to 100 ml of a Tris-albumin-based extender (v/v). Each tube was then divided into six aliquots according to the assessment frequencies. The spermatozoa concentration was 75 × 10^6 per 0.25 mL. Progressive motility, viability, and morphology were assessed at 0 hours and every two hours up to 10 hours after dilution and storage at 27°C. The statistical significance of the results was assessed using a one-way analysis of variance (ANOVA). significantly different results were further tested with Ducan’s test with SPSS version16.0 software. The results indicate that the mean percentages of progressive motility in FF0, FF1, FF2, and FF3, measured 10 hours post-extension and preserved at 27ºC, were 45.04±2.74, 43.04±4.18, 47.05±5.70, and 53.80±1.74, respectively. The mean percentages of sperm viability were 48.09±3.15, 50.52±2.50, 50.12±2.83, and 57.88±3.85, respectively. The mean percentages of abnormal sperm were 15.21±3.26, 14.15±2.16, 13.03±1.11, and 12.12±1.22, respectively. The results of the study suggest that the addition of 8 ml (v/v) of tris-albumin-based extender has a significant preservative effect on all three indicators: progressive motility, viability, and morphology of spermatozoa, making it suitable for artificial insemination (AI) for up to 10 hours after dilution and storage at 27°C. It would be advantageous to conduct more comprehensive research to investigate the effects of varying FF concentrations in different storage conditions, such as room temperature, refrigeration, and freezing, on semen preservation. This would not only be beneficial for AI programs, but also contribute to the existing literature on semen preservation extenders.
Keywords | Tris, Albumin, Fig fruit filtrate, Spermatozoa, Goat
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