Phenotypic and Genotypic Characterization of Pasteurella Species Isolated from Camels in Egypt
Phenotypic and Genotypic Characterization of Pasteurella Species Isolated from Camels in Egypt
M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2*
ABSTRACT
The main aim of this study is to apply phenotypic and genotypic characterization of members of Pasteurella species isolated from camel from different governorates in Egypt. Camels with respiratory manifestation as nasal discharge, fever, off food, with ocular discharge and air gasping, paralysis of lips and salivation were subjected to clinical examination and a total of 141 samples were collected as follow; 111 nasopharyngeal swabs, 15 tracheal swabs and 15 lung specimens collected from camels from different governorates (New valley, Giza, Naj Hammadi (Qena governorate), Halaib and Shalateen (Halaib Triangle) were subjected to isolation and identification of Pasteurella species by conventional phenotypic method and genotypically by polymerase chain reaction (PCR). At the level of biochemical characterization, the incidence of Pasteurella multocida was 40.4%, and Mannheimia haemolytica was 7.1%. Using KMT1 gene for identification of the isolates for P. multocida and SSE gene for identification of the isolates for M. haemolytica, the results revealed that six isolates showing positive PCR for Pasteurella multocida and were subject to further phylogenic characterization. M. haemolytica could not be detected by PCR. Identification and characterization of Pasteurella species isolated from camel improve diagnosis, epidemiology and treatment of the organism causing diseases.
Keywords | Camel, Pasteurella multocida, Mannheimia haemolytica, Phenotypes, Genotyping, PCR
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