Antiviral Potential of Ivermectin against Peste des Petits Ruminants Virus (PPRV)
Antiviral Potential of Ivermectin against Peste des Petits Ruminants Virus (PPRV)
Saba Afzal1, Sohail Raza1,*, Masood Rabbani1, Sehrish Firyal2, Imran Altaf1 and Zahra Naeem1
ABSTRACT
This study was conducted to find the antiviral potential of ivermectin against Peste des Petits Ruminants Virus (PPRV) in vitro. Firstly, cell cytotoxicity of ivermectin in the Vero cells was determined using MTT assay. The antiviral activity of ivermectin was determined using viral inhibition assays and median tissue culture infectious dose (TCID50). The results showed that 2.5 µM concentration of the ivermectin is non-toxic to the Vero cells. At this concentration, PPRV titre was significantly reduced (p < 0.001) by two log to 103.0 TCID50/0.1 mL as compared to virus control 105.5 TCID50/0.1 mL. Moreover, the ivermectin exposure after the viral attachment and entry steps able to reduce virus titre more as compare to viral attachment and entry steps that predicts the possible mechanism of the drug. Therefore, our study first time demonstrated the antiviral potential of ivermectin against PPRV in vitro and its future potential for its use as anti-PPRV therapeutics.
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August 2021
Vol. 53, Iss. 4, Pages 1201-1601
