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Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum Stunt Viroid Egyptian Isolate from Infected Chrysanthemum Plants

Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum Stunt Viroid Egyptian Isolate from Infected Chrysanthemum Plants

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

  Virology Lab., Agric. Microbiology Department, Faculty of Agriculture, Ain Shams University, 11241 Cairo, Egypt.
  Plant Virus and Phytoplasma Research Section, Plant Pathology Institute, Agriculture Research Center, Giza, Egypt.
  Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
*ahmd sofy@yahoo.com

ABSTRACT

Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (⁓199) was cloned into the PCR Il TOPOvector then it was sequenced. Partial sequence 199 bp of CSVdEG is almost identical to that of the prototype 199 bp Canada and USA isolates of CSVd with 96% homology. The sequence of CSVd-EG can be arranged into viroid specific rod like structure. CSVd-EG differ from the prototype isolates Canada and USA at sites occur in regions corresponding to the conserved, variable and right terminal domains which are believed to control viroid pathogenicity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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