THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
Muhammad Akram and Faheem Aftab
ABSTRACT
In the present study, axillary shoot bud induction, the growth of shoot and callus formation was observed. Shoots were cut to prepare nodal explants (5-10 mm long), surface sterilized and inoculated on MS (Murashig and Skoog, 1962) medium supplemented with different concentration (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1,2,3,4 or 5uM) of TDZ for 24 days. Highest bud break (100%) was obtained at 0.005 uM TDZ after 14 days of initial culture. The same cultures were further maintained and subsequently obtained with 20.6 mm shoot length and 7.2 average number of leaves for another 10 days. Similarly, all other TDZ’s concentrations below 1 uM were also effective in bud breaking and development of shoots with green and vigorous growth. The frequency of bud break was significantly reduced with the increasing concentrations of TDZ. There were 2 phases of callus formation on the explant, i.e., before bud break and after the break. The frequency of callus induction was highest (100%) at 5uM TDZ before bud break along the nodal region. The rate of callus formation was significantly reduced once bud began to break down and initiation of green axillary shoots. The shoots were further developed, rooted and acclimatized to glasshouse conditions. We demonstrated that axillary buds of G. biloba could be initiated and proliferated with TDZ under in vitro conditions.
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