Genetic Analysis and Phylogenetic Study of IL-10-Like Protein and ORF103 Encoded Genes of Sheep Poxvirus Infecting Sheep from Iraq
Genetic Analysis and Phylogenetic Study of IL-10-Like Protein and ORF103 Encoded Genes of Sheep Poxvirus Infecting Sheep from Iraq
Mohammed A. Al-Saadi1*, Yahia I. Khudhair1, Muthanna Hadi Hussain1, Monyer Abdulamier Abd Alfatlawi2, Mourad Ben Said3
ABSTRACT
This study aimed to investigate the genetic and phylogenetic characteristics of IL-10-like protein and ORF103 encoded genes of Sheep poxvirus (SPV) in sheep in Iraq. DNA samples of three sheep farms with clinical signs of pox infection were employed to collect the specimens from Al-Qadisiyah Province, Iraq, and the DNA was extracted and amplified by PCR targeting IL-10 and ORF103 genes. After purifying the PCR products, the amplicons were sequenced by Sanger method. Following the sequencing, the Data were studied using the MEGA11 software. The findings revealed that infected sheep showed typical pox lesions in various parts of the body. while lambs infected with SPV, showed more severe symptoms of this infection. The genetic analysis of both IL-10 and ORF103 genes showed high sequence similarity with sequences from Turkey and Saudi Arabia. This study provides information about the molecular characteristics of SPV after the genetic analysis of the important genes of this virus. This gives us ideas to develop better diagnostic tools and control measures and help to decrease the effect of this infection on sheep populations. These findings continue to emphasize the importance of doing a continuous genetic monitoring of SPV on field, that will help us to keep the animal health status, in addition to controlling outbreaks.
Keywords | Cytokines, Dermatitis, Itch
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November 2024
Vol. 12, Sp. Iss. 1
