Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...se (FMD) remained an endemic disease in Pakistani livestock population during 2011 and 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene i...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...se (FMD) remained an endemic disease in Pakistani livestock population during 2011 and 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene ...
Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
...tic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data on the overall genetic diversity, true origin and complex evolutionary patterns of human RVA strains. In this review, we have summarized and discussed the significance of recent research outcomes obtained from whole genomic analysis of common and unu...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...se (FMD) remained an endemic disease in Pakistani livestock population during 2011 and 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene i...
El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...gypt has experienced endemic outbreaks of avian influenza viruses (AIV) of subtype H5N1 in poultry since 2008. Since then, these viruses continue to evolve. In 2006-2008, outbreaks were reported mostly during the winter season, but in 2009-2012 they occurred year round. H5N1 AIV succeeded in infecting not only domesticated birds but also many other avian species and mammals. Infections of humans were accompanied by mutations in the hemagglutinin (HA) protein w...
Joseph Andrew Bracken
Metaphysics for a World in Evolution
...ch the Many by their dynamic interrelation from moment to moment initially co-create and then sustain the One not as a higher-order individual entity but as their conjoint energy-source and ongoing field of activity. The One can then be readily integrated into a hierarchical ordered system of fields within fields at all levels of existence and activity within nature. In the final part of the article, I indicate how this new field-oriented understanding of the ...
Muhangi Denis
...mpacts on the socio-economic status of farmers. The disease is endemic in a number of countries in sub-Saharan Africa, in Sardinia (Italy), the Russian Federations, the Caucasus and it has shown possibilities of spread within Eastern Europe. Involvement of all stakeholders in the pig value chain in designing and implementing of prevention and control strategies could be explored in future control programs. This may call for ...
Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1
...te of the art of CDV dynamics in South America, by means of a detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating strains....
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...y and caused severe economic losses to the poultry industries. H9N2 AIVs can evade the pressure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human epidemics. The threat to the poultry industry and public health should not be ignored. In...
Aradhana Chopra1, Ravi Shukla2 and Tarun Kumar Sharma2,3*
...hat are considered as chemical rival of antibodies. They are generated through an in vitro evolution process called SELEX. These single stranded nucleic acid aptamers can acquire various secondary and tertiary structures to bind their cognate targets with high affinity and specificity. The ease in synthesis and modification makes them an attractive candidate for a variety of applications. Since the last decade, aptamers have been regarded as an emerging player...
Nicola J. Stonehouse
...the context of a sub-genomic replicon and highlight the future potential of this technology in many aspects of virus research as well as in diagnostics and therapeutics....
Justin P. McBrayer
...ons for belief are epistemic in nature. Some of our reasons for belief are prudential in the sense that believing a certain thing advances our personal goals. When it comes to belief in God, the most famous formulation of a prudential reason for belief is Pascal’s Wager. And although Pascal’s Wager fails, its failure is instructive. Pascal’s Wager fails because it relies on unjustified assumptions about what happens in the afterlife to those ...
Mark Zelcer
...ook is principally a polemic against inserting metaphysical assumptions into mathematics, science, and religion. The book focuses on the metaphysical worldviews of philosophers, scientists, mathematicians, and religious thinkers. Though for reasons I elaborate on later, I would not recommend this to experts in those fields, but rather to non-professionals....
Justin P. McBrayer
...ons for belief are epistemic in nature. Some of our reasons for belief are prudential in the sense that believing a certain thing advances our personal goals. When it comes to belief in God, the most famous formulation of a prudential reason for belief is Pascal’s Wager. And although Pascal’s Wager fails, its failure is instructive. Pascal’s Wager fails because it relies on unjustified assumptions about what happens in the afterlife to those ...
Xingdong Yang and Lijuan Yuan
...ntained free of unwanted microbiota in sterile isolators. The sterile surgical derivation and gnotobiotic status of the pigs allow studies of the infection, disease and immune responses caused by a specific pathogen in the absence of interfering maternal antibodies, other maternal immune regulators, and intestinal and environmental microbes. Due to the similarities between pigs and humans in terms of genetics, physiology, in...
Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3
...ruminants (PPR), an economically important morbillivirus infection of sheep and goats, is widely distributed in Middle East, Central and South Asia, China and Africa. Whereas PPR antibodies were first reported in Kenyan small stock as early as 1995, the clinical disease was observed later in 2006 following dramatic and devastating PPR outbreaks in Turkana County. In this context, an outbreak of PPRV was observed in Tukana County in 2011 and the current study d...
James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...ngue and malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to initiate the appr...
Khalid Usman, Aziz-Ur-Rehman
...ystems. However, N agronomic efficiency, grain yield and net benefit were similar for all three tillage systems. Regarding N application timing, T3 had highest values for N agronomic efficiency, grain yield and yield components. Results also suggested that application of N in three splits particularly in T3 is more productive and economical strategy for wheat production following cotton. I...
Salma Shaheen, Muhammad Jamil Khan, Saleem Jilani
...ic manure and complex chemical fertilizer (NPK) with or without effective microorganism (EM) on the agronomic performance of spinach crop, at Gomal University D.I. Khan during 2009- 11. A commercial product called Bio-Aab was used as a source of EM. Organic manure was first mixed with Bio-Aab, left for 7 to 15 days at room temperature (20oC) and then applied to appropri- ate pots. The trea...
Shahid Ali, Munir Khan
...r wheat crop, labour, chemical fertilizer and tractor plough would raise the wheat yield by 0.052, 0.566, 0.130 and 0.438 percent, respectively and were found statistically significant. Farmers’ education was found to be major determinant of technical efficiency/inefficiency. The esti- mated coefficient of farmers’ education was negative and statistically significant, implies that techni- cal inefficiency decreases with the increase in farmers&rsqu...
Mohammad Fayaz, Abbas Ullah Jan, Dawood Jan
...ousehold Integrated Economic Survey (HIES) data from Pakistan Social and Standards Living Measurement (PSLM) 2010-11. The elasticities of interest were obtained via log-log inverse (LLI) functional form of Engel equation. The quantity and expenditure elasticities remained less than unity with reasonable differ- ence in magnitude across urban and rural households. Quantity and expenditure elasticities of all the vegetables were higher for rural households than ...
Farhad Zulfiqar, Muhammad Ashfaq
...anatory variable (eco- nomic, location and climatic) from 1979-2009 relating to wheat were used for Faisalabad and Ba- hawalpur. The results of analysis showed that the effect of climatic variables was found significantly higher than that of non-climatic variables. The largest impact on wheat yield was of mean maximum average temperature at the time of maturity, ceteris paribus with one oC increase in temperature the average wheat yield increased by...
Stephen Suleyman Schwartz
...r are not aspects of Islamic history. To declare, as even French president Francois Hollande did, “these terrorists and fanatics... have nothing to do with the Muslim religion” is inaccurate....
John Teehan
...again, the threat of Islamic radicalism to the foreground of public consciousness. Such attacks, of course, raise vital questions of public safety and the costs we are willing to pay for that safety. They also raise questions of how to understand the complex conditions that give rise to such events and the conditions for such events are always complex, even when facile “explanations” are readily available, i.e. the violent nature of Islam, their ha...
Lawrence Krauss
...tests throughout the Islamic world to the bittersweet cover published the week following that tragedy....
Ghazalah Yasmin1*, Mir Ajab Khan2, Nighat Shaheen3, Umbreen Javed Khan4
E-mail | [email protected]
Sarhad Journal of Agriculture, Vol. 31, Iss. 1
... three (A. alpinum, A. rumicifolium and A. tortuosum) of five species distributed in Pakistan using light microscopy and scanning electron microscopy. Results showed that Aconogonon is characterized by tricolpate pollen with microspinulose type of exine ornamentation. One distinctive pollen type was recognized on the basis of aperture number and sculptur...
Yashab Tur
Science, Religion and Culture, Vol. 2, Iss. 2
...d the opening view panoramic: Imagine a world still living with centuries-old routines and patterns of individual and communal life, submerged in the slow rhythm of a pre-modern era, about to encounter modernity! This is the world that Elshakry describes in her groundbreaking work, a world which encounters cylinder-powered platen printing machines, the telegraph, the steam ships and railways—all coming from countries dimly perceived and barely understood...
Thomas J. Coleman III
Science, Religion and Culture, Vol. 2, Iss. 2, Pages 39-41
Masood ur Rahman1*, Zahid Mahmood2, Taj Ali2, Muhammad Aziz Irfan Mufti3, Jehangir Khan Seyal4, Munir Ahmad5
E-mail | [email protected]
Sarhad Journal of Agriculture, Vol. 31, Iss. 1
...were determined using atomic absorption. The gears under test exhibited significant difference in surface wear among each other. As compared to gear bronze commercial gun metal and gun metal showed surface wear of 245% and 109% respectively. The highest surface wear was observed in commercial gun metal whereas lowest surface wear was observed in gear bronze. It was concluded that gear bronze may be the best material composition for use in the worm gear of the ...
Suresh V Kuchipudi* and Kin-Chow Chang
...influenza and other systemic viral infections which warrants further in-depth investigations.
...
Hu Zenglei1 and Xiufan Liu1, 2*
..., which is currently endemic in many countries of Asian and Middle East, replicates at a significantly higher level, induces more potent innate antiviral and inflammatory response, and causes more severe damages in lymphoid tissues when compared with virulent viruses of other genotypes. Therefore, severe pathology in immune organs, caused by genotype VIId of NDV, is associated with high levels of virus replication and an intense innate immune response....
Malik Ikram Ullah1, Abdul Aziz Khakwani1*, Muhammad Sadiq1, Inayatullah Awan1, Muhammad Munir2, Ghazanfarullah1
...ion and quality and economic return of fodder maize cultivar Kissan. The experiment was conducted on silt loam soil at Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan during 2011. Six nitrogen treatments (80, 120, 160, 200, 240 and 280 kg ha-1) and a control one were studied in this trial. Growth and yield traits and quality such as plant height, stem diameter, leaf area plant-1, leaf area index, chlorophyll content, green fodder yield, dr...
Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*
...by phosphorus mobilizing microorganisms 2.5 packets ha-1, in addition to control were evaluated under lentil-sorghum and lentil-mungbean cropping patterns. Fertilizer treatments were only applied to lentil while their residual effect was studied on sorghum and mungbean. The results revealed that net benefits and marginal rate of return from NPK were the highest mainly due to high lentil yield and poultry manure was next to it. The residual analysis ...
Huaichang Sun
...is the use of artificial microRNAs (amiRNAs) to fight virus infections. However, different strategies are needed to prevent virus variation or mutation escape. This review is intended to present the current situation of antiviral roles of amiRNAs, against variable RNA viruses in particular....
Josef D. Jarhult1, 2
...n important part of pandemic preparedness plans. Resistance development is a potential problem both in treated humans and in the environment. The use of oseltamivir for treatment of uncomplicated influenza in otherwise healthy patients should be questioned....
Khalid Usman1*, Niamatullah Khan2, Fazal Yazdan3
...strength (28.5 g tex-1), micronaire (4.3µg inch–1) and uniformity (80.2%) were also higher for DNH-105 than other varieties. Ginning out turn ranged from 37.2 (CIM-612) to 39.9% (DNH-105). Fibre length ranged from a low of 28.3 (CIM-612) to a high of 29.2 mm (DNH-105) while fibre strength was observed 25.0 (CIM-612) to 28.5 (DNH‑105) g tex-1. Micronaire value ranged from 4.3 (DNH-105) to 4.8 &
Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1
...hids was achieved at 80 µg mL-1 and the LC50 for the fruit body extract after 24 HRS was calculated as 12.83μg mL-1, followed by the extract from the fermentation filtrate with LC50 value 25.03 μg mL-1. The mycelial extract with LC50 (29.96 μg mL-1) exhibited least insecticidal activity. The results obtained from this study revealed that these extracts possess metabolit...
Imdad Sohu1, Allah Wadhayo Gandahi2*, Ghulam Raza Bhutto2, Muhammad Salim Sarki2, Rabail Gandahi3
.... All the relevant agronomic observations and chemical analysis of the soil and plant samples were recorded. The application of organic sources of farmyard manure and poultry manure in combination with inorganic NPK fertilizers had shown positive effect on chickpea plant height, number of branches plant-1, number of pods plant-1 and seed index. The growth and yield of chickpea significantly increased with the collective app...
Benjamin Beit-Hallahmi
...red by most humans. Academic research, or science, is the product of counter-intuitive, unnatural psychological processes, and the resulting concepts are beyond the reach of most. It is not surprising that religion has been around for possibly more than 100,000 years, while academic research is a recent historical development. Over the past century, individuals who make academic research t...
Marcus Mann
...as the first of four polemics that have come to symbolize what is now known as “The New Atheism,” a term meant to encapsulate a 21st century religious skepticism characterized by its vitriolic and unapologetic stance toward religion and the moral relativism that the new atheists claim protects it. Following Harris’s example, evolutionary biologist Richard Dawkins published his book, The God Delusion, cognitive philosopher Daniel Dennett came ...
Ghulam Ali Bajwa and Muhammad Arshad Khan
...gn: justify;">Macro- and micro nutrients were managed in soil and mulberry foliage through application of urea and diammonium phosphate (DAP). Urea at the rate of 608 kg/ha and DAP at the rate of 260 kg/ha was applied on six mulberry varieties. The results showed a deficiency in nitrogen, phosphorus, zinc and manganese in soil before the fertilizer application. Contrarily, high copper and potassium contents were found. After three weeks of fertilizer applicati...
Ali Muhammad*, Muhammad Ayub
... were analyzed physicochemically. Result showed that data is significantly different from each other at alpha (α) level ≤ 0.05. Physicochemical analysis showed that decrease was observed in ascorbic acid (15.18 to 7.21), pH (4.56 to 3.9) and TSS (5 to 2.5). Standard deviation showed the least variation in data. Results show that on nutritional basis RW1 was found best followed by RW2....
Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah
...contain several allelochemicals. Utilization of these allelochemicals is considered low-cost and natural sources for weed suppression. Field trials were undertaken at Cotton Section, Agriculture Research Institute, Tandojam Pakistan during summer 2010 and repeated during summer 2011 for validating the previous results. The experiments were laid out in three replicated randomized complete block design. The...
Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*
E-mail | [email protected]
...viridae. JEV is endemic to many parts of East Asia, Southeast Asia and Australia, where periodic outbreaks take thousands of lives. With rapid globalisation and climatic shift, JEV has started to emerge in previously unaffected areas. Scientific evidences show that JEV would remain a global pathogen and might cause worldwide pandemics. Currently, more research is required to be applied to JEV pathogenesis and antivirus ...
Brittany D. Rife 1,2, Marco Salemi 1,2*
E-mail | [email protected]
...wed similarly as an epidemic spread on a microscale, creating a unique opportunity for the application of phylogeography at the intra-host level, which has until recently been limited by dataset quality and the lack of a rigorous statistical framework. As recently described in studies using the SIV-macaque model of neuroAIDS, the incorporation of
spatial, temporal, and demographic inference into intra-h...
spatial, temporal, and demographic inference into intra-h...
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...indicate that BTV is endemic in sheep herds from Costa Rica, and animals seem not show clinical signs. We recommend carrying out further studies, to determine the presence of BTV in goats and wild ruminants, and to identify serotypes present in the country....
Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2
Email: [email protected]
...species were Culicoides imicola (94.2-95.85%) and Culicoides newsteidi (2.21-2.72%). The rate of prevalence of these species was bi-phasic and higher from April to June, and October to November 2009. Interestingly, the infectivity rate of BTV was also higher during these periods indicating that high risk of infection is directly proportional to that of transmission vectors. Moreover, results demonstrate that the life stage “bumper&rdq...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: [email protected]
...gy and medicine. Metagenomics is one of these emerging NGS applications and offers the opportunity to study i.e. whole ecosystems. Basically, the metagenomics approach is similar to well-known shotgun-sequencing, though on a much bigger scale. For instance, the metagenome of a lake would include all the fish, ducks, plants, fungi, bacteria and everything else that belongs to the lake. If we apply this approach to clinical sa...
Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3
...s promising potential as microbial control agent against A. sciodoxa and should be exploited under field conditions....
Kang-Seuk Choi
... viral capsid proteins mimic the naive configuration of authentic IBD virus particles. The VLPs show intrinsic immunogenicity and a high safety profile. Thus, VLPs are considered one of the most promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential of VLPs as an alternative to IBD vaccines, along with their specific applications i...
Seyed Mohsen Mirbod
...ich is easy to use, economical and effective. A new knee orthosis was designed to improve the performance of OA patients during standing and walking. Two subjects with knee osteoarthritis recruited in this research study. The gait performance of the subjects and their stability to stand was evaluated by the use of motion analysis system and a force plate (Kistler). The mediolateral excursion of the knee joint decreased while walking with the knee orthosis...
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
...etection and disease dynamics. The validity and the reliability of generated data can be enhanced significantly appropriate measures. As a standard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several p...
Muhammad Amjad Nadim1*, Mohammad Safdar Baloch1, Ejaz Ahmad Khan1, Abdul Aziz Khakwani1, Kashif Waseem2
...ynthetic fertilizers and micronutrients on growth and yield of wheat was conducted at the Agricultural Research Institute, Dera Ismail Khan, Pakistan. The trial was laid out in a randomized complete block design (RCBD) using split-plot arrangements with four replications. Main plot consisted of NPK and organic fertilizers while micronutrients viz. Zn, Cu and B (alone and in combinations) were assigned to sub-plots. The resul...
Khalid Ali1*, Amanullah Jan2
...mproving soil physico-chemical properties and fertility. Two years field experiments were conducted at the Agriculture Research Institute Tarnab, Peshawar- Pakistan (semiarid conditions) to investigate the effect of two green manuring crop species (guar and millet), their incorporation age (40, 70 and 100 days after sowing, DAS) and parts (whole plants incorporation and stubbles incorporation) under varying nitrogen levels (0, 75 and 100 kg ha-1) on physiology...
Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2
...s in MFSC were the agronomic practices whereas there was big gap in horticultural practices. Major constraints identified were less machinery utilization duration, complication in machinery booking process, costly rental prices, out-dated machinery, preference of progressive farmers, no timely availability of Inputs, unviability of crop specific machinery. It was recommended that MFSC may take initiative to compel private companies to sponsor schemes for low c...
Abiodun D. Olabode
...nformation on socio-economic activities of the rice farmers, level of rice yield, agricultural practices for rice production and farm management techniques employed in the study area were observed. The study employed simple percentages to analyse the farmers’ responses; while regression model established significant relationships between rice yield and farm management methods adopted in this area. Results were presented using charts and tables. The study...
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...ffinity by an ELISA-like microplate-screening assay (ELASA). Top three candidate aptamers were then paired in a matrix of all possible capture and reporter aptamer combinations and tested in a fluorescent peroxidase-linked Amplex® UltraRed (AUR; a resazurin-like substrate) version of a rapid (< 1 h) aptamer magnetic bead sandwich assay. The optimal sandwich aptamer combination utilized the same aptamer sequence (designated Rt-18R) for both capture and r...
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
...stan and is becoming endemic in many regions. The first case of CCHF was reported in 1976 in Rawalpindi and after that, multiple sporadic cases and outbreaks have occurred in subsequent years and till today. CCHF outbreaks have a case fatality rate of up to 40%, which is alarming. It is a zoonotic disease and the virus is transmitted mainly to the humans through hard tick bite and direct contact with blood of the infected animal. The occurrence of CCHF outbrea...
Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4
...ission and evolution dynamics of PPMV-1....
Moustafa Kardjadj
...and one of the most economically devastating diseases that affect cloven-hoofed livestock including cattle, goats, sheep, pigs and wild ungulates. Wide prevalence of the disease in Asia and Africa associated with huge economic losses to livestock farming and industry has increased global concern for the disease. Currently, 3 serotypes of FMD virus (O, A and SAT-2) and 06 lineage are circulating...
Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
...also to estimate the dynamics of viral population in the infected hosts. Several recent methodologies and chemistries have been proposed for viral diagnostics with variable outcomes and successes. In this review, we discuss a novel approach to identify the least transcripts of viral genomes using suppression subtractive hybridization that can be adapted to unanimously identify viruses of diverse genetic backgrounds. <...
Murtaza Ali*, Yasser Durrani and Muhammad Ayub
...d evaluated for physiochemical and sensory parameters (water activity, ash content, percent acidity, pH, moisture content, reducing and non-reducing sugars, taste, texture, color and overall acceptability). Statistically minimum decrease was observed in water activity, pH, moisture content and organoleptic characters. While, stable increase was noted in percent acidity and ash content during storage. It was concluded and recommended on the basis of mentioned f...
Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2
...erosis for various agronomic and biochemical traits in Flue Cured Virginia tobacco. Seven tobacco varieties/lines, ‘NC606’, ‘K399’, ‘Spt G 126’, ‘Spt G 28’, ‘KHG21’, ‘KHG22’ and ‘KHG24’ with contrasting traits were crossed in all possible combinations to generate 7 x 7 diallel crosses. In 2008 and 2009, all F1 hybrids along with th...
Ashley C. Banyard1* and Satya Parida2
.../i> virus causes an economically important disease of small ruminants that causes extensive livestock losses across areas where it is endemic. Further the recent description of the disease in naïve populations of animals have demonstrated an expansion of the distribution of the disease. Having been in the shadow of rinderpest virus for decades little is understood regarding factors that influence the outcome of infectio...
Vinayagamurthy Balamurugan*, Gurrappa Naidu Govindaraj and Habibur Rahman
...ous, notifiable and economically important transboundary viral disease of sheep and goats. PPR is enzootic in India as more number of outbreaks have occurred in the past and now occurring regularly, round the year and most frequently during the lean period throughout the country. In some Indian states viz. Andhra Pradesh, Karnataka, and Chhattisgar h the PPR outbreaks in sheep and goats have declined after implementing the strategic mass vaccination programme....
Moustafa Kardjadj1, Pam Dachung Luka2
...us (PPRV) causes an economically important plague of sheep and goats that serves as a major constraint in the development of the small ruminant’s production within an infected area. The virus is endemic across much of the developing world and has spread into the developed European borders. The small ruminants industry within an infected state or region is often disproportionately affected with its attending impact on p...
Gurrappa Naidu Govindaraj, Vinayagamurthy Balamurugan*, Habibur Rahman
...ade to estimate the economic losses based on the annual incidence, morbidity, mortality levels etc. derived from literature, discussion with experts, and based upon scientific facts. Different mathematical models were used to assess the losses due to mortality in young and adult sheep/goats, body weight loss in young and adult sheep/goats, milk loss, loss due to increased inter-lambing/kidding period, loss due to increased abortion, cost of high feeding and re...
Neil Levy
...ribute to valuable epistemic diversity. ...
Neil Levy
...ribute to valuable epistemic diversity. ...
Keyvan Sharifmoradi1*, Nader Farahpour2
...ercentage to improve dynamic stability and decrease the magnitude of destabilizing forces. The mean values of propulsive component of anteroposterior force and the second peak of vertical ground reaction force decreased significantly due to performance of ankle joints plantar flexor which decreased in this group of subjects....
Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
...ants. The disease is endemic in Bangladesh and causes considerable economic losses due to its high morbidity and mortality. The study was undertaken to detect and characterize the PPR virus (PPRV) circulating in Bangladesh. Twelve local isolates of Bangladeshi PPRV were successfully detected from post mortem samples (lymph node, spleen and trachea) of goats and a sheep during the year 2008-2012. The partial N gene of ten iso...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...tial to underpin the endemic potential of PPRV and its possible spread to the susceptible wild or domestic small ruminants....
Muhammad Siddiq1* and Hamid Ullah Shah2
...nd in terms of physicochemical properties between olive and palm oils. Laboratory experiment on “Effect of stress conditions on the quality and stability of olive and palm oil blends” was conducted at Nuclear Institute for Food and Agriculture (NIFA) Tarnab Peshawar and The University of Agriculture Peshawar KP-Pakistan during 2015. Olive: palm oils treated in sole or admixture at various ratios were kept at different stress conditions for three mo...
Shahab e Saqib, Mokbul Morshed Ahmad, Sanaullah Panezai, Hidayatullah, Khalid Khan Khattak
... of important socio-economic factors in access to credit. The data were collected from 87 farming households in Mardan Tehsil of Mardan District. Questionnaire was the main tool used to collect the data from farmers. The credit access ratio formula was employed that measured farmers’ access, in relative terms, to their landholdings. Likewise, credit adequacy formula was used to investigate farmers’ credit adequacy. A probit model was employed to fi...
Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan
...d Mn concentration. Economic analysis showed that NPK75FYM20 rewarded the maximum net income of Rs. 43108 ha-1, indicating its superiority for profitable wheat yield under rain-fed conditions. It can be concluded from these results that the application of plant nutrients from a variety of natural and manufactured fertilizer sources improve crop yield as well as augment the fertility of soils suffering from water erosion hazards....
Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid
... Pakhtunkhwa by using atomic absorption spectrophotometer. In all the three districts the egg albumen was found to contain significantly higher levels of Pb, Cd and Cr as compared to egg yolk which contains significantly higher levels of essential elements (Fe, Mn and Zn). The mean concentrations of toxic heavy metals; Pb, Cd and Cr in albumen samples collected from Peshawar were 0.13, 0.06 and 0.09 (ppm) respectively. Concentrations of Pb, Cd and Cr in egg al...
Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad
... mean (1.79±0.033µm) SL compared to Nili Ravi (1.81±0.039µm). It was concluded that beef from Kundi buffalo is relatively better in beef quality characteristics (Marbling, Water holding capacity, Sarcomere length, and Tenderness) than Nili Ravi buffalo. Kundi buffalo may be further selected for improvement in beef quality traits and could be a potential source of quality beef for export in future.
Abdul Qahar and Bashir Ahmad
...mum grain yield and economic benefits....
Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker
... that enforce major economic threats to the livestock industry throughout the world. The present cross sectional study was conducted to assess the prevalence of tick in goat’s population of three different areas (Kaptai, Kattoli and Chittagong Veterinary and Animal Sciences University) of Chittagong, Bangladesh. A total of 60 goats were examined for tick infestation in three places using random positive sampling technique. Among the breed, Black Bengal g...
Rashid Mahmood1*, Saima Asad2, Waqar Ahmad1, Ghulam Sarwar1, Muhamamd Khalid Rafique1, Noor Islam1, Ziyad Abdul Qadir1 and Zain Ul Abiden2
...), thymol (0.5 g) and formic acid (65%) on screen bottom boards in winter, spring and summer seasons. The fourth group had screen bottom board trays but without any chemical. There was a trend of fallen mean number of mites 157±6.28, 97±3.81, 132.75±9.76 and 26±1.68 in winter, 160±20, 149±30, 161.75±18.5 and 27.5±1.04 in spring and 188.25±5.34, 196.25±5.48...
Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2
... using scanning electron microscopy. The physical parameters such as weight and swelling degree of composite were studied. Chitosan starch based composites were used in treatment of waste water to remove a toxic anionic dye Congo red. Congo red solution (0.015 mM) was treated with polymer composite at neutral pH overnight with 150 rpm orbital shaking at room temperature. The same procedure was performed for commercial dyes. The dye was removed due to the inter...
Mian Shamas Murtaza1, Aysha Sameen1, Nuzhat Huma1 and Fatma Hussain2
... analyzed for physico-chemical composition, functional, textural and sensory quality. The results showed that reduction in fat significantly (p < 0.05) increased the moisture and protein content. The addition of gums further augmented the moisture level owing to owing to their water retention properties. The melt-ability, flow-ability and yield of cheese decreased significantly (p < 0.05) on reducing the fat level, as anticipated. However, the addition o...
Allah Wadhayo Gandahi, Khalilluah Panhwar, Rabail Gandahi, Muhammad Saleem Sarki and Mahmooda Buriro
...ha-1 and 5 secondary and micronutrient (Control, 50, 3, 4 and 1.5 kg ha-1 Mg, Fe, Zn and B, respectively) treatments. NPK @ 120-70-40 kg ha-1 resulted maximum plant height, number of green leaves plant-1, number of opened bolls plant-1, seed index, and seed cotton yield ha-1. Red leaves reduced when soil was fertilized with N, P and K @ 120-70-40 kg ha-1; and plots without application of K (120-70-0 kg ha-1) and PK (120-0-0 kg N ha-1) increased number of red l...
Muhammad Khurshid, Muhammad Nafees, Inam-ur-Rahim and Wajid Rashid
...madic life to socio-economic and environmentally vulnerability in upland areas....
Hassan Tarik Atmaca
...ghly contagious and economically devastating viral disease of small ruminants and camels, and thus is negatively affecting the livelihoods of poor people in many developing countries. Efforts are being made to advance our understandings on different aspects of molecular biology of the virus and to consolidate national and international strengths to eradicate the disease from the planet. This Editorial highlights recent research articles and reviews that have p...
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...illium muscarium with chemical nematicides, Vydate, Basamid (G) and fertilizer, Calcium cyanamide through soil application to control sugar beet cyst nematodes Heterodera schachtii. Lecanicillium muscarium decreased nematode population significantly (P=0.05 and caused reductions in final numbers of cysts per 250 g of soil, eggs and juveniles per cyst. Nematicides effects were not quite significant among each other but had tremendous effects in reducing nematod...
Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
...monly used plants in Islamic countries.The root extracts of S. persica has been tested for its anticoccidial activity against Eimeria paillata induced infection in mice jejunum. Three different doses, 300, 600 and 900 mg/kg were used after infection of mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mic<...
Wan-Long Zhu* and Gao Wenrong
...cal, hormonal, and biochemical markers indicative of thermogenic capacity. The results showed that A. chevrieri adapt to winter or under cold condition by increasing thermogenesis and food intake. The present results suggest that the observed physiological regulation from the organismal, hormonal levels to the cellular levels of this alpine small mammal were important and allowed A. chevrieri to overcome the physiological challenges of an change ...
Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa
...using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine nor urea and were preliminarily identified as members of the Mycoplasma mycoides sub-cluster which comprises Mmm and Mycoplasma mycoides subspecies capri (Mmc). Three (3) o...
Muhammad Aslam
...onstraints include agronomic, irrigation management, environmental, technological, institutional and socio-economic constraints. Future prospects include upscaling of modern technology, enhanced seed production, improved inputs availability and use, improved irrigation, improved agriculture-education-training-research- extension-nexus, reclamation of salinized lands, improved agricultural credit and support price policies. R...
Mazhir Nadeem Ishaq, Li Cui Xia, Rukhsana Rasheed, Zeeshan Ahmad, Muhammad Abdullah
...mpts to measure the economic benefits of milk marketing cooperatives working in two districts (Vehari and Muzzafargrah), geographically located in the southern region of Punjab province of Pakistan. The study analyzed the significance of milk marketing cooperatives by revealing and evaluating possible returns to smallholders in dairy sector. The hypothesis that milk marketing cooperatives are beneficial for milk producers was tested by surveying the cooperativ...
Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah
...y and diabetes.Hypolipidemic and hypoglycemic effect of water based infusion of Withania somnifera was investigated in broiler chicks. One hundred and twenty (120) day-old broiler’s chicks were alienated into four treatment groups namely WST-0, WST-I, WST-II and WST-III with three replication. Group WST-0 was kept as a control, while WST-I, WST-II and WST-III were treated with W. somnifera water based infusion @ 10, 20...
Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*
...porting the latest taxonomic arrangement by IUCN....
Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...ed. Moreover these biochemical parameters were confirmed by histopathological examination using hemotoxylin and eosin (H&E) and Mason trichrome stains (MTC). Immunohistochemical investigations for the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels....
Mehreen Zaidullah and Hina Fatima
...ousehold Integrated Economic Survey for the years1985-86, 1993-94, 2001-02 and 2007-08. Quadratic Almost Ideal Demand System (QUAIDS) is employed to estimate the budget shares and test for these shifts. The results confirm for the significance of the quadratic variables in the model except for the Oils’ group. The presence of these shifts have been proved in Pakistan and hence there are other factors as well like urbanization that causing these shifts in...
Shagufta Jabeen1, Muhammad Saqlain Raja1, Sadia Saeed1, Muhammad Mobeen Zafar1, Rizwana Abdul Ghani1, Abid Mahmood1, Muhammad Fiaz2, Pakeeza Arzoo Shiaq1, Shahid Mahmood Baig3, S.M. Saqlan Naqvi1 and Ghazala Kaukab Raja1*
...edical, social, and economic problems arise. Drug addiction is among major health issues faced by the World including Pakistan with an alarming annual increase in heroin addicts. The study was designed to identify the socio-demographic risk factors contributing to increasing susceptibility to heroin addiction in Pakistani populations. In this epidemiological study subjects were interviewed through a comprehensive questionnaire consisting of open and closed-end...
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
...nt diseases causing economic losses to dairy farm owners. In order to develop new strategies to prevent mastitis, a detailed understanding of the molecular mechanisms underlying the host immune response to the infectious agents is necessary. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify potential genetic markers for resistance to mastitis. Thi...
Aqila Shaheen and Mehwish Matien
...C storage and physico-chemical properties mainly in relation to climate, soil type and vegetation....
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*
...ere sequenced, their genomic structure examined, and their genome organization, arrangement and codon usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii
Determination of Early Colonizer Urban Blowfly Species Using Small Bait Traps on a University Campus
Halide Nihal Açikgöz1*, Serdal Kenan Köse2 and Ali Açikgöz3
....24%) in 2015. Macro and micro climatic factors and biodiversity of the fauna are significant for solving suspect entomological cases.
...
Ai Ming Zhou1,2, Guang Wen Liang2, Ling Zeng2, Yong Yue Lu2 and Yi Juan Xu2*
...invicta (Hymenoptera: Formicidae) suppress ghost ants Tapinoma melanocephalum (Hymenoptera: Formicidae) through interference competition. However, relatively less testing have been done to evaluate the competition for mutual exploitation between the two ant species in the field. Here, we investigated the interference of the fire ant S. invicta on the interactions between the ghost ant T. melanocephalum and the invasive mealy...
Haji Muhammad, Zafar Iqbal* and Saira Saleemi
... by using standard taxanomic keys on the basis of morphometric characters. Seventy species belonging to 43 genera and 17 families were recorded from the study area of Indus River. Thirty two species were commercially important. Alpha Diversity Indices study showed that fish diversity of the River was quite rich (species richness, 70) and diverse, (Shannon’s index=3.66), (Simpson-D=0.96), Evenness was also high (Evenness (H/S) =0.55) and Chao-1 (70.75). T...
Sadiq Hussain, Muhammad Sharif, Sarmad Khan, Fazli Wahid, Hina Nihar, Wiqar Ahmad, Imran Khan, Nadeem Haider and Tabassum Yaseen
...hiza inoculation with vermicompost on yield and P uptake of wheat. The experiment was carried out in Completely Randomized Design replicated four times during season 2014-15. Phosphorus (P) was applied at recommended level of 90 kg ha-1. Vermicompost was used as a source of P on the basis of its P concentration. The recommended dose of N @ 120 kg ha-1 was applied in the form of vermicompos...
Irfan Ullah and Abbas Ullah Jan
...ffects of important economic factors such as prices and income. Flexible Linear Approximate Almost Ideal Demand System model is used to estimate food demand patterns using the Household Integrated Economic Survey of Pakistan for the year 2011-12. Food products are categorized into fourteen groups including milk, meat, fruits, vegetables, sugar, beverages, wheat and wheat flour, rice, other cereals, pulses, oil and fats, tea ...
Zaheer Ahmad Qureshi and Khuram Nawaz Sadozai
...use Hold Integrated Economic Survey (HIES) collected by Pakistan Bureau of Statistics to obtain the income and expenditure level of sampled House Hold. The HIES data set of year 2011-12 for KP Province was comprised of the total of 1352 sampled House Hold. However, the sampled rural House Hold was 926. The statistical framework comprised of Lorenz curve and Gini Coefficient to gauge the income inequality in the study area. To assess the income inequality both ...
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...or inhibit the growth of microorganisms. Their abuse results in various side effects on the human health, environment and agriculture. The occurrence of bacterial “suprainfection” with tolerance to antibiotics has attracted the significant attention, mainly due to the consequences of multi drug resistance and potential threat to human health and the environment. With the increasing incidences of antimicrobial con...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...gi on the population dynamics of this pest in sugar beets in laboratory and greenhouse trials. The fungi chosen were Arthrobotrys oligospora, Dactylella oviparasitica, Clonostachys rosea, Stropharia rugosoannulata, and Lecanicillium muscarium. In the laboratory experiment, S. rugosoannulata proved to be the most efficient biocontrol agent by parasitizing the maximum number of eggs, whereas D. oviparasitica appeared to be the least efficient after 72 hours...
Syeda Farzana Bibi*, Lal Badshah and Siraj-ud-Din
Muhammad Basit1, Shafqat Saeed2, Mushtaq Ahmad Saleem3, Rana Zulfiqar4
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...rmine the population dynamics of the root knot nematode, Meloidogyne incognita, and the nematophagous fungus, Lecanicillium muscarium, on tomato. The nematode population densities were measured by determining the number of galls and egg masses, juveniles and eggs per root system, and reproduction factor. Four initial populations (Pi) (500, 1000, 1500, and 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×1...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...ium muscarium and the chemical nematicides Vydate and Basamid (G) with the fertilizer calcium cyanamide in the control of root knot nematode, Meloidogyne hapla, through soil application under greenhouse conditions. A significant (P=0.05) reduction in the number of galls and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all che
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
...trols were examined. Genomic DNA was extracted and amplified by PCR using specific primers for GPR54 and GNRHR splice site exons. Mutations were analyzed by single-stranded conformation polymorphism (SSCP) and/or sequencing. No mutation was identified in GPR54 gene, while two mutations in GNRHR gene were observed in one sporadic case of isolated HH. The first was a synonymous substitution mutation of T to G at nucleotide position 123, which did not replace val...
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...study aims to source dynamic ecological and epidemiological field parameters to describe a slum household-based pathogenic Leptospira population dynamics simulation model. The model will be analyzed to assess the role of slum-based biotic (human and animal) and abiotic (soil, water and weather) habitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have ...
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
... resource with high economic value. Somatostatin (SS) is a neuropeptide family which effects growth, development and metabolism. In this study, full-length of one type of SS cDNA from C. nasus was synthesized, cloned and sequenced. This SS cDNA encodes a protein with 114 amino acids that contains the SS14 sequence at its C-terminus. This putative peptide is identical to that generated by the SS1 gene in other vertebrates. Tissue distribution of C. nasus SS1 mR...
Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
...e results of the phytochemical analysis showed that the extract has high polyphenolic content while has relative low flavonoids contents. In the antiradical scavenging property test the extract showed at 64.1 μg/ml 50% DPPH radical scavenging activity and in the MTT assay the extract has relatively low cytotoxicity where the IC50 value of extract against Vero cells was 1078.69 µg/ml. Furthermore, the extract exhibit...
Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4
...eneficial and pathogenic microbes and its pH (6.7 to 7.4), high concentration of phosphorus (4.50% and 4.33%) and nitrogen (3.26% and 2.37%) favor seed germination, enhance root growth and soil fertility....
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...uctural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could be obtained. Recombinant Npm or PGA was injected into porcine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than t...
Muhammad Sohail1,*, Muhammad Naeem Khan1, Naureen Aziz Qureshi2 and Abdul Shakoor Chaudhry3
... were exposed to none (0µg L-1), low (120 µg L-1), medium (240 µg L-1) and high (360 µg L-1) levels of lead (Pb), chromium (Cr) and copper (Cu) alone and in combinations (Pb + Cr + Cu) for 15 days under laboratory conditions. Gill cells of mussels were used to determine the DNA damage by comet assay. The tail DNA (%), comet tail l...
Jose M. Rojas, Noemi Sevilla and Veronica Martin
...ue to the important economical losses caused by this virus in Africa and Asia, the development of more effective, targeted and successful treatments for the disease are necessary. Like other Morbilliviruses, PPRV induces a suppression of the immune system during the acute disease, which favours the establishment and exacerbates the progression of secondary infections. This review gives an overview of the known immunosuppressive strategies of PPRV, among the Pa...
Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6
...te production status, chemical composition, and fish species being used for producing fish meal. The overall production of single processing unit ranged from 40 to 500 metric tonns per month. Kampa Industry (Unit 1) was found to be the first largest contributor producing 100 metric tons in 24 h. The second largest producers of fish meal in this region were Abdul Baqi, Ghulam Hussain and Kampa Industry (Unit 2) which produced 300 to 500 metric tons per month. P...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
... malaria infection. Systemic lupus erythematosus (SLE) is an autoimmune disease that affects many body organs including spleen. However, the effect of Plasmodium chabaudi infection on the spleen of the murine lupus model BWF1with the comparison between live and gamma irradiated parasite was not studied before. A total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus un...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...rimarily at the hypothalamic level of the gonadotropic axis. Citric acid is synthesized and secreted by the prostate gland. Its synthesis is regulated by androgens. As, dose dependent degeneration of prostate gland has been described following intraperitoneal kisspeptin treatment. However, effects of kisspeptin administration on the levels of prostatic citric acid remain elusive till date. The present study, therefore, addresses the effects of 12 day administr...
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...wed that the H9N2 is endemic and widely distributed in asymptotic layers. Furthermore results indicated that H9N2 subtype may survive in layers without showing any symptoms....
John G. Bruno
...assessed by fluorescence microplate assessment following acridine orange staining. While there was significant cross-reactivity between the various captured serotypes, each of the anti-LPS aptamer candidates showed a preference for its cognate E. coli serotype. Results suggest that these aptamers may be useful for enriching specific E. coli pathogens during aptamer-magnetic separation in food samples, leading to a greater ability to detect these serotypes if c...
Zafar Waheed, Khalid Usman, Iftikhar Ali
...t was conducted at Agronomic Research Farm, Faculty of Agriculture, Gomal University, Dera Ismail Khan in randomized complete block design having three replications during 2013-2014. The treatments comprised of wheat + Rumex dentatus 0 plants m-2 (T1), wheat + Rumex dentatus 05 plants m-2 (T2), wheat + Rumex dentatus 10 plants m-2 (T3), wheat + Rumex dentatus 15 plants m-2 (T4), wheat + Rumex dentatus 20 plants m-2 (T5), wheat + Rumex dentatus 25 plants m-2 (T...
Muhammad Shafiq and Tahir Maqsood
...pplication. Regarding chemical analysis concentration of boron in both wheat straw and grains increased with B application but there was no effect of fertilizer B on NPK concentration of straw and grains. These results are very heartening; signifying that for any soil, B adsorption isotherm need to be worked out for location precise fertilizer B use....
Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi
....27 ±17.77 cells/µl. Out of 278 subjects tested, 11 (4%) were positive for anti-EBV IgM. Anti-EBV IgM was highest in the age group 41-50 years, 5 (5.81%); females, 8 (4.85%); students (14.21%); and CD4+ 350-500 cells/µl, 4 (36.36%). The multivariate analysis using age and the CD4 count showed highest prevalence (27.27%) among the age ranging from 41 to 50 years with the CD4+ count of 350- 500cells/&
KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder
...sease causes severe economic loss due to reduced productivity of the affected animals as well as increased mortality in calves and kids. In this study proportionate prevalence of FMD in cattle was estimated and the distribution of FMD according to different factors was evaluated. Both retrospective and prospective FMD clinical cases were included considering clinical signs and drugs used for treatment during 13 January to 15 March 2015. A total of 131 cattle w...
Sezgi Arman* and Sema Isisag Ucuncu
...sent study, some histochemical features of goblet cells of the intestine of convict cichlid (A. nigrofasciata) were described. In order to reveal the main histological construction, transverse sections of different parts of intestine were firstly stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-S...
Sezgi Arman* and Sema Isisag Ucuncu
...sent study, some histochemical features of goblet cells of the intestine of convict cichlid (A. nigrofasciata) were described. In order to reveal the main histological construction, transverse sections of different parts of intestine were firstly stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-S...
Ibrar Khan1,2,3,*, Sadia Qayyum2, Shehzad Ahmed2, Kashif Syed Haleem2, Mujaddad-ur-Rehman1, Guang-Lei Liu3 and Zhen-Ming Chi3
...the most common and economically significant group of marine micro-fungi family. The study comprised isolation, microscopic observations and ribotyping, allowed discrimination of the marine fungal isolates as Penicillium viticola F1, Penicillium restrictum F2, Penicillium rubens F8, Penicillium implicatum F10, Penicillium piceum F11, Penicillium oxalicum F12, Penicillium sumatrense F15, an...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...bbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. In this Experiment, we established a unique muri...
Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*
...eir morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. Their sensitivity against three phages indicated broad host range of phages SANF and SA2, and relatively narrow host range of phage SA. Phage SNAF showed an effective growth reduction of S. aureus RP isolate, compared with other bacteriophages. The bacterial challenge test in milk i...
Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*
... most studied and deconomically important crustaceans in China. However, the production scale does not meet the market demand. This resulted in excessive fishing, which almost caused extinction of the species in the the Yangtze River. Therefore, it is very urgent to carry out genetic breeding to improve yield. In this study, we developed SSR markers by transcriptome, which could be used to genetic research. In this study, we performed transcriptome sequencing...
Oluwasogo David Olorunfemi, Oluwasegun Adetokunbo Adekunle, Felix Olayinka Oladipo, Temitope Oluwaseun Oladele and Oladimeji Idowu Oladele
...e fish farmers were economically active with a mean age of 42.5 years, majority (73.1%) were males, married (74.4%) and had one level of formal education or the other (82.5%). Chi-square analysis revealed a significant relationship between the respondents’ training needs on value addition initiatives and their age, educational level, years of experience and fish farm income. The value addition initiatives use profile of the fish farmers was still very lo...
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
... and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant from HWE in RA group. The results of present study indicates that Pb and Cr concentration differ significantly between AA, AG and GG genotypes of SOD1 (rs2070424) gene pol...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
... physiological and biochemical characterization. 16SrRNA sequencing was performed to identify three highly resistant strains at species level. Antimicrobial activity of aqueous and methanolic plant extracts of Camellia sinensis (Green tea), Syzygium aromaticum (Clove) and Mentha piperita (Peppermint) was evaluated against identified isolates. Agar well diffusion assay was used to monitor the antimic<...
Bin Wang, Bo Zhang, Nan Yang, Liang Dou and Jianghong Ran*
...hasis szechenyii) is endemic to western China and is known to cooperatively breed in a population habituated to supplemental feeding by humans. The social structure and demography of this species, however, have not been examined in natural populations. To determine if cooperative breeding occurs in populations unassociated with humans, we surveyed two natural populations in the Ganzi Tibetan Autonomous Prefecture in western China. We compared the demography of...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
...us (PPRV) causes an economically important disease of small ruminants that causes extensive livestock losses across areas where it is endemic. A serological survey on the prevalence of antibodies to PPRV was carried out in sheep and goats of Jatoi and surrounding areas in Muzaffargarh districts South Punjab of Pakistan. A total of 965 serum samples were collected from sheep and goat’s farms from January to December 201...
Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran
...ditions....
Neelum Andaleeb and Munir Khan
Qamar Ali, Muhammad Ashfaq and Muhammad Tariq Iqbal Khan
...e sheet, fertilizers, chemical, irrigation and labour days. Under utilization of resources was found for polythene sheet, fertilizers, chemicals, irrigation and labour days. Over utilization of resources was observed for seed quantity and use of tractor. There exist decreasing return to scale (87.7%) but its value will surely increase after efficient use of inputs. A little adjustment is required in the use of inputs for rec...
Muhammad Jamal Nasir, Anwar Saeed Khan, Said Alam and Saeed Akhtar
...ductivity and socio economic condition of the residents....
Muhammad Aslam Shahid, Muhammad Iqbal and Muhammad Niamatullah
...et, yield and physico-chemical characteristics of Dhakki date palm. Data were recorded for spath length, width, number of strands/spath, number of flower per strands, number of spath per tree, weight of pollen grain/spath, weight of pollen grain/tree, pollen viability, fruit set, yield and physico-chemical characteristics. The results indicated that males were significantly differed from one another in all these characters d...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
...s fungal species for antimicrobial natural products. During this study five different fungi, including Alternaria alternata, Acremonium sp., Alternaria brassicicola, Pythium sp. and Aspergillus flavus were screened against Wilt causing pathogens using the dual culture assay. Among them, Aspergillus flavus was found active fungus against the selected vascular Wilt causing fungus and bacteria, i.e. Fusarium oxysporum (87.50 ± 0.11% inhibition), Xanthomona...
Zaffar Malik, Moazzam Jamil, Ghulam Hassan Abassi, Muhammmad Nafees, Muhammad Rafey and Muhammad Kamran
...he availability and economic level of the landholders....
Muhammad Afzal1 and Murad Khan2*
...: justify;">To make economic analysis of broiler poultry farms, a survey research was carried out in district Lower Dir in 2015. Four tehsils were selected namely Adinzai, Balambat, Timergara and Sumerbagh on the basis of purposive sampling technique. From these tehsils, 92 farms were selected randomly from 460 poultry farms. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, descriptive statistics and gro...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 ceramide in promoting apoptosis. The cytotoxic effects of these components cause ultrastructural changes in A549 cells.
...
S. Bushra1,*, M. Tariq1, M. Naeem1, M. Ashfaq1, I. Bodlah1 and M. Ali2
...ant extracts and semiochemicals on physiology and performance of endoparasitoid of aphids, Diaeretiella rapae. Seven different treatments of semiochemicals and plant extract were applied on aphid pests, Sitobion avenae and Rhopalosiphum padi and parasitoid was released. The data regarding emergence, parasitism, sex ratio, tibia length, adult weight and adult longevity of parasitoid D. rapae after ...
Wan-Long Zhu1 and Guang Yang2,*
...ing the role of hypothalamic neuropeptides genes expressions on body mass regulation under different photoperiods in Eothenomys miletus, body mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), Agouti related peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that short photoperiod reduced...
Effect of Supplemented Algal Carotenoid Diets on Skin Color of Tomato Clownfish, Amphiprion frenatus
Muge Aliye Hekimoglu*, Kgrşat Fırat, Şahin Saka, Cüney Suzer, Aysun Kop and Yaşar Durmaz
... to be 0.77±0.61 µg.g-1 on the initial day in the experimental group. As a result of the measurements performed on the 120th day, the pigment values were determined as 30.39±0.39 µg.g-1 in Group A, 39.07±1.12 µg.g-1 in Group B and 35.68±10.69 µg.g-1...
Irshad Ahmad
...s the cautious use of chemical insecticides, mechanical means, cultural and biological control methods for the substantial control of walnut borer. The present study offers valuable information through IPM, which appears to be a promising approach for the control of walnut borer. Collectively this strategy has resulted in significant reduction of the pest population in the target area of district Dir, Pakistan.
...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...ing an integrative taxonomic approach that combines morphology and molecular analysis of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Adult P. websteri exhibit a high degree of morphological plasticity in the palp pigmentation pattern, the shape of the anterior edge of the prostomium, the shape of the major spines on chaetiger 5, and the shape of the pygidium. The COI gene sequence demonstrated that the intraspecific distance of P. we...
Muhammad Mohsin Javed*, Muhammad Azhar Nisar, Bushra Muneer and Muhammad Usman Ahmad
...ext-align: justify;">The microbial fuel cell (MFC) was constructed from polypropylene random (PP-R) pipes jointed in U-shaped manner with a cation exchange membrane. The MFC constructed was subjected for the bioelectricity production by using vegetable waste extract as substrate. The power production was analyzed for three days. Maximum power density of 88990mW/m2 with a current density of 314.4mA/m2 was observed on the second day of proc...
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
...the reproduction of economically domestic animals. Some microsatellites related to the fecundity of domestic animals have been identified. In the current study, we investigated five fecundity related microsatellites, including BM1329, BM143, LSCV043, OarHH35, and OarHH55, which had been identified in sheep. We estimated their diversity and population structure in three high fecundity goats...
Mahanama De Zoysa1, Si-yun Ryu1, Hyeon-cheol Kim2 and Bae Keun Park1*
...ht and scanning electron microscopy.
...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
...ricultural crops of economic importance.
...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 µM 5-Fluorouracil...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
...s including eggplant. Chemical control of nematodes is hazardous to health and causes environmental pollution by contaminating underground water. The bio-protectant potential of mycorrhizal fungus (Glomus mosseae) and neemex® (Azadirachtin) against invasion and development of Meloidogyne incognita was tested in eggplant roots in greenhouse pot trials. Neemex (5 g, 10 g and 15 g) and G. mosseae (100 g, 150 g and 200 g) were applied as p...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...d subsequently their antimicrobial susceptibility.The milk samples were screened with surf field mastitis test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
...(ng/mL), T4 (µg/dL), TSH (μIU/mL), and kisspeptin (ng/ml) was done. BMI and hemoglobin were significantly reduced (P<0.001), while serum ferritin were significantly increased (P<0.001) in all four thalassemic groups on comparison with control. Kisspeptin levels were significantly reduced in ≤13 years female (P<0.001). While significantly high levels were observed in >13 years of ...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...tological and bile biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the m...
Rashid Ahmed Khan* and Muhammed Naveed
...rence and population dynamics of the fruit fly were compared with meteorological factors, such as temperature, relative humidity and sunshine. The highest mean population of fruit fly remained at 499 in the month of August. It started to decline afterwards and remained at 348 in the month of September. The lowest population was recorded at 26, 3, 2 and 1 for November, December, January and February, respectively. The optimum monthly average temperature and rel...
Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
...stant cultivars for economically important crops through molecular breeding techniques via utilizing advanced molecular markers involving cis and trans genetics. The review adhere recent research findings regarding disease resistance against viral diseases and concludes via shedding light upon the future prospects in this exciting field of research.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...urvivors, 162 Normolipidemic healthy controls and 102 patients with hypercholesterolemia in a Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT geno...
Zafar Iqbal1* and Muhammad Khurshid2
...ere losses for many economical important crops in tropical and subtropical regions of the world. This is the first report of the use of heterologous polyclonal antibodies (PAbs), raised against a geographically distinct begomovirus, ToLCNDV. The described results have shown to exhibit a many fold increase detection sensitivity and reproducibility hence, can be adopted for routine detection of ToLCNDV from plant extracts.
...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
...ens with subsequent economic losses to poultry industry. Effected birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of av...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...he light on emerging antimicrobial resistance that represent major concern for public health. A total one hundred swabs and 500 organ samples were collected from apparently healthy and freshly dead quails respectively. Bacterial isolation and characterization were performed in accordance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds all f...
Abdul Wajid Khalil and Zafar Iqbal
...plusmn;0.08g/100g), Glutamic acid (25.27±0.06 g/100g), Alanine (12.74±0.12g/100g) was found in higher concentration in aerial parts than roots while some essential amino acid, Tryptophan (6.23±0.06g/100g), Arginine (3.49±0.08 g/100g), Phenylalanine (1.77±0.08 g/100g) were recorded higher in roots as compared to the aerial parts of B. procumbens. It is concluded that aerial parts may serve as a supplement for human diet as wel...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
...the serotypes while Gentamicin had the lowest resistance (5.9%).This is one of few studies on the occurrence and antimicrobial resistance profile of Salmonella in poultry slaughter slab (processing plants) in Nigeria. The relatively high prevalence rate documented in this study may be attributed to the generally poor infrastructure, lack of well-equipped poultry slaughter houses, lack or inadequate water supply at these mark...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
...y;">Mankind is using antimicrobial agents in various forms since last 2000 years. The present study was designed to evaluate the antimicrobial activities of different culture microbes against each other. A total six bacteria including Salmonella typhi, Escherichia coli, Pasteuralla multocida, Lactobacillus bulgaricus, Micrococcus luteus and Staphylococcus aureius were used ag...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Rassol Bakhsh Tareen2, AsadUllah1 and Zahid Mustafa1
...on in all three forms of microbial population. On the other hand, herbs added as feed additive acted as prebiotic and enhanced the lactic acid bacteria. In conclusion, supplementation of herbs as feed additive improved growth parameters and present results suggests herbs could be used as alternative to antibiotics growth promoting feed additives.
...
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...nd transmission electron microscopy. Numerous degenerated follicles with cytoplasmic vacuolization, mitotic catastrophe in mitochondria , chromatin condensation, mitochondrial vesiculation and dispersion at ooplasm were observed. In mitochondria, mitotic catastrophe , vesiculation, swelling and loss of organization of cristae were detected.Here we showed that TiO2 exposure trigger paraptotic type cell death in zeb...
Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*
...e associated with the chemical ecology of the Asian Long Horn Beetle (ALB), Anoplophora glabripennis. It further provides a brief impression of ALB’s biological characteristics, ecology, chemical ecosystem, economic significance, and management. Beetles in the Cerambycidae family have implicit increasing importance as pests of green forest in addition to shade trees, shrubs, a...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
...: justify;">Porcine epidemic diarrhea (PED) was triggered by pathogenic coronavirus causing watery diarrhea and high mortality in piglets. This post-viral syndrome results in severe economic losses in the pig industry. However, limited information on the porcine epidemic diarrhea virus-infected pigs is available. Thus, the objective of this study was to identify transcriptomes with RNA seq...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...enus-specific bcsp31 genomic region based rPCR was used. This was followed by two species-specificrPCRs that detected IS711 genomic region of B. abortus and B. melitensis. Five (8.3%) serum samples from cattle and 1 from buffalo (1.6%) were found to be positive for B. abortus by RBPT. Four (6.6%) out of the 5 RBPT positive cattle samples were also found to be positive by i-ELISA, while one was neg...
Irum, Akram Shah*, Sobia Wahid, Nazma Habib Khan and Qaisar Jamal
...re evaluation along with microscopic assessment of water samples for Cyclops species. Based on previous clinical records of dracunculiasis, five villages of Dera Ismail Khan including Kath Garh, Wanda Dost Ali, Chah Mundar Wala, Ketch and Gara Baloch were surveyed for a total of 206 randomly selected households, where the household heads (106 males and 100 females) were interviewed for any previous dracunculiasis lesion scars or active infections among ...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...nths age), in rabies endemic Lahore city of Pakistan, to evaluate their maternal rabies virus neutralizing antibody titer (mRVNA). All puppies were whelped by dams regularly vaccinated with high quality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppie...
Habib Ullah Khan1*, Saleem Ullah1, Hamid Ullah Shah1, Muhammad Arif2
...to 11.9 and 3.45 to 28.4µs/dl respectively. Soil EC was lower 96.60 µS/dl in untreated soil while highest 190.10 µS/dl in NSP treated soil. Acidity of the treated soil ranged from 0.97g/L in S to 1.41 g/L in FN treated soils. The highest value (6.58x10-3%) of total nitrogen was in CFNSP treated soil. An increase in soil pH was noted where treatments variation ranged from ...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...ast and are causing economic losses in the commercial poultry production sectors. Due to potential of H9N2 infections in human, LPAI H9N2 poses serious public health risk. Therefore, development of new potent and cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was to develop H9 plasmid-based DNA vaccine against the currently circulating AIV subtype H9N2 targeting HA gene of the v...
Waqar Islam
...ir damage caused to economically important crops are reportedly increasing every day particularly in the countries embedding tropical and subtropical conditions. Integrated management approaches involving utilization of virus resistant crops and efficient management of insect vectors can reduce this disastrous problem. However, in developing countries such strategies are rarely applied due to lack of farmer’s knowledge about plant virus diseases. Therefo...
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...es were subjected to genomic DNA extraction to detect Mycoplasma spp. using multiplex-PCR. Isolation and identification data showed that a total of 58/96 samples (60.41%) were positive for Mycoplasma infections whereas a total of 61/96 blood samples were also found positive using serum plate agglutination. However, the multiplex-PCR detected Mycoplasma in all of the tissue samples, either one of the species or simultaneous infection. M. gallisepticum was found...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...enetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating the elevated risk in this cosmopolitan cities. Phylogenetic analysis indicates that M. m. musculus migrat...
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...roups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopreservation solution. After 2 months, MTT assay showed significant differences in the proportion of adherent viable cells in the FH group and GSH group compared with the control group (p < 0.05), and the FH+H group had a more b...
Adel Eid Mohamed Mahmoud*
...were observed in meat chemical analysis of slaughtered animals among different groups. Animals fed BBP recorded insignificant increase in growth rate by 9 g daily with improvement in feeding cost by 10%.Bakery waste can replace corn grains and wheat bran in concentrate feed mixtures up to 60 % in growing lambs rations without any adverse effects on animal’s performance with increasing growth rate.
...
YaQiu Liu and ZhiJian Wang*
... with light and electron microscopies. Intestinal tract was divided into three parts. Morphologically complex folds were formed on surfaces of anterior and middle intestines where many secretory cells were present. Highly developed junction complex were seen in anterior intestine. Cytoplasm contained abundant mitochondria and pinocytotic vesicles. Epithelium of posterior intestine was thin, translucent and squamous with dense blood capillary network undergroun...
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...se (AD) involve rats and mice, yet the tree shrew (Tupaia belangeri) is in many respects more similar to primates than rodents are. To demonstrate the feasibility and usefulness of using the tree shrew as an AD model, we examined how well the model recapitulates features of the disease. Injecting animals intraperitoneally with d-galactose to induce acute senility, and then injecting them with β amyloid fragment 1-42 and ibotenic acid into the bilat...
Sanaullah Jalil*, Asim Hayat, Abid Majeed, Syed Haider Abbas, Muhammad Noman, Muhammad Imran Kasana and Muhammad Mazhar Hussain
..., E0 = without effective microbes and E1= EM-Biozote @ 50 l ha-1) on yield parametrs of wheat crop planted after the harvest of rice. All yield parameters significantly increased by the treatments consisting of RP (100 kg) with FYM (5 t ha-1) and EM @ 50 l ha-1, which also enhanced the nutrient uptake of the crop. It can be concluded that accurate use of cheaper rock phosphate, farm yard manure and effective microbes proved ...
Response of Spring and Autumn Sunflower to Chemical and Bio-Fertilizers for Yield and Quality Traits
Muhammad Tahirand Memoona Shehzadi*
...flower by using the economical and environment friendly techniques. Present study was proposed to evaluate theresponse of spring and autumn sunflower to chemical and bio-fertilizers for yield and quality traits during 2014 at University of Agriculture, Faisalabad, Pakistan. The treatments comprised on the combination of chemical fertilizers (No chemical ...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
...E), wheat yield and economics, a field experiment was carried out at Arid Zone Research Center, Dera Ismail Khan, Khyber Pakhtunkhwah Province, Pakistan, during rabi season of 2014-15 and 2015-16. The experiment was laid in randomized complete block (RCB) design with split plot arrangement keeping pulverization viz. single tiller P1, double tiller P2, triple tiller P3 and mould board plow P4 followed by single rotava...
Wazha Mugabe1,2, Lawrence Akanyang1, Mackenzie Nsinamwa1, Batanani Moatswi1, Naledi Matthews1, Kealeboga Dipheko1, Imtiaz Ahmed Ujjan3 and Assar Ali Shah2*
...us understanding the dynamics on rangeland response to grazing could prove worthy in finding an equilibrium point for optimizing animal productivity, with limited range degradation. Therefore, the current study was aimed at determining and comparing fodder tree species composition along grazing gradient in fenced and unfenced grazing area in the Gaborone North Region. For each study area, two parallel transects measuring 1000 m radiating from the water points ...
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
... extract having 968.125 µg/ml of chlorogenic acid inhibited significantly the growth of tested bacterial strains. Among the bacterial strain tested Salmonella entritidis and Helicobacter pylori were even more sensitive to 20.313 (µg/ml) of chlorogenic acid and showed zone of inhibition with the value of 6.0 and 7.0 mm. The HPLC analysis of apricot suggested that 5.0 gm of apricot contain 1.04 &
Ahmad Khan1, Raza Ullah Khan1*, Muhammad Zameer Khan1, Fayyaz Hussain1,Muhammad Ehsan Akhtar2, Jalal-Ud-Din3
...evaluate the effect of humic substances (HSs) derived from coal, sunflower, and maize waste materials on onion yield. Four HSs rates; 0, 20, 30 and 40 kg/ha were applied to onion (variety Red Swat) transplanted earlier to field (plot size=1.2×0.8 m2). Results showed that maximum bulb yield of 25.1 and 24.5 t/ha were obtained with application of plant derived HSs; SFDHSs and MDHSs respectively, while 24.7 t/ha bulb were obtained with CDH...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...ng transmission electron microscopic and light microscopic results were found: degenerative changes in the liver tissue, significant decreased in the number of activated Bcl-2, while increased in the number of Bax and caspase-3-positive hepatocytes, a significantly increase in levels of the activities of biochemistry markers (serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP)). In c...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...oducts will be thermodynamically stable. The sequence-based predictions suggested that the pseudogenes-derived proteins may involve in different biological functions like translation, energy metabolism, amino acid metabolism and transport and binding.
... 
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...sed on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molecular (nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies available to virologists have obviously become more diverse and improved, the challenges have also changed greatly.Detection of plant viruses is becoming more critical as globalization of trade,particularly in hor...
Sitansu Pan and Amrita Das
Evaluation of shelf life of some value added organic formulations of Trichoderma harzianum
...nic formulations like vermicompost, leaf manure, rice bran and FYMand also their combinations with two oil cakes, neem and mustard cake. The growth was estimated in terms of colony forming unit (c.f.u.)/g of substrate up to 120 days at 30 days interval. In all organic products or their amendment with oil cakes c.f.u. increased up to 60 days and then gradually declined. Among the four organic media, vermicompost retained 21.3...
Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram
Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
... for isolation of chilli microflora. From these samples, 94 fungal and 89 bacterial isolates were derived, which included 50 isolates of fungi and 44 isolates of bacteria from phylloplane and pomoplane, 44 isolates of endophytic fungi and 45 of endophytic bacteria from inside the leaf and fruit tissues, respectively. Among them, 70 fungal isolates belonged to plant pathogenic genera like,Alternaria, Cercospora, Colletotrichum, Curvularia, Glomerella, Mycosphae...
Wajid Hasan1 and C. P. Singh2
Relationship between the population of mustard aphid,Lipaphis erysimi (Kaltenbach) and weather parameters on different cultivars of Indian mustard ( Brassica juncea L.)
...igate the population dynamics of the mustard aphid,Lipaphis erysimi Kalt. on different 42 cultivars of Indian mustard (B. juncea). The results revealed that the population of L. erysimi exhibited negative correlation with maximum and minimum temperature,rainfall, wind velocity, evaporation and positive with afternoon and morning relative humidity. The values of coefficient of determination (R ) were high (0.92 to 0.99), indicated that the population of L. erys...
1N. Johnson Singh, 2Amitava Konar and 3Palash Mondal
Incidence of aphids in different traps on potato in Gangetic plains of West Bengal
...luate the population dynamics of aphids with the help of yellow pan trap and yellow sticky trap. The population of aphid catches was quite lower in yellow pan trap than yellow sticky trap. Aphids were first observed in traps during third week of December and the population of pests gradually increased to reach the peak in second week of February in the first year of study (2006-07) while in second year of study (2007-08) the aphid population appeared in second...
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...ely affecting beneficial microbes like Rhizobium and capable of promoting growth of certain crops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production is highly labour intensive and fit for cottage industry. These products are used mainly for direct s...
1S. A. Khan and 2S. Jha
Effect of dates of sowing, moisture regimes, varieties and weather factors on incidences of aphid, Lipaphis erysimi (Kalt.) in rape and mustard
...and aphid population dynamics during different phases of growth of rainfed vis-à-vis irrigated rape and mustard crops, and work out relationships between aphid population and weather parameters for its forewarning. Results revealed that initial incidence times of aphid varied from 19 November to 3 December, depending upon dates of sowing done during 1 October to 5 November, which fall under recommended dates of sowing. In crops sown during 22 October to...
1Bikash Ranjan Ray and 2Mrinal Kanti Dasgupta
Management of root holoparasite Aeginetia pedunculata of (Orobanchaceae), causing wilt of sugarcane by trap and catch crops
.... (Orobanchaceae) is endemic in the command area of Plassey Sugar Mill in the districts of Nadia and Murshidabad in West Bengal. It spreads through water-and soil-borne seeds. With the objective of developing location-specific integrated management schedule of the parasite, crops, other than sugarcane, grown in the target area were evaluated for their potential as trap and catch crops of the parasite. Twelve crops, selected on the basis of germination inductio...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...ontrol (P< 0.05). The microorganisms which caused high nematicidal activity also showed antifungal activity in dual plate culture test in vitro. All the antagonists showed reduction in root knot and root rot disease and promote plants growth as the length and weight of root and shoot in pot trials on tomato plants. Keywords: Antagonistic fungi, root knot nematode, wilt fungus, bio-management
...
S. Subharani, S. S. Thorat, N. Abem, L. Amit Kumar and T. K. Singh
A database on parasitoid of insect pests of crops in Manipur, India
...The database is for academic purpose and it includes the taxonomic details of the parasitoid, host insect, host plant, morphological characters, geographical distribution, period of activity, parasitoid behavior and its biology etc. Image of the parasitoid species are also provided for helping in easy identification of the species. Keywords: Database, insect-pest, parasitoids, Manipur
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Sitansu Pan and Someshwar Bhagat
Biological control of plant diseases
...hat is why consortium of microorganisms is needed for tackling several plant pathogens simultaneously instead of only one biocontrol agent-one pathogen,which is the most common and established system of biocontrol of plant diseases....
Sitansu Pan and Amrita Das
Population proliferation and spread of Trichoderma spp. in soil under two different delivery systems
...hrough organic based (vermicompost + neem cake (90% + 10% w/w)) and seed based (sterilized wheat seed) inocula. The population proliferation and spread of the antagonists were faster when they were applied through organic based inoculums over that of seed based inoculums from the point of application. Similarly population proliferation and spread of the antagonist was more rapid in sterilized soil followed by solarised soil and natural soil respectively. Among...
K.N. Ahmed , M.A. Al-Helal, N-E-P. Khanom, S. Bulbul
Control strategies of papaya mealybug, Paracoccus marginatus Williams & Willink infesting vegetable crops in Bangladesh
...plants and weeds of economic value. The papaya mealybug feeds on the sap of the plants by inserting its stylets into the epidermis of the leaf as well as into the fruit and stem. In doing so, it injects a toxic substance into the leaves. The result is chlorosis, plant stunting, leaf deformation, early dropping of the leaves and fruits, a heavy accumulation of honeydew and death of the host plants. Heavy infestation is capable of making fruits inedible due to t...
M. S. A. Mamun and M. Ahmed
Integrated pest management in tea: prospects and future strategies in Bangladesh
...tea. Extensive use of chemical pesticides began only a few decades ago with tremendous immediate economic gains but its abuses were not foreseen or ignored. As a consequence there arose the development of resistance to pesticides, pest resurgence and undesirable pesticide residue in the made tea as the major problems. Current trends in eco-friendly insect pest management practices emphasize the host plant resistance, prepara...
Sitansu Pan and Amrita Das
Control of cowpea (Vigna sinensis) root and collar rot (Rhizoctonia solani) with some organic formulations of Trichoderma harzianum under field condition
... of the antagonist in vermicompost along with 20% neem cake (w/w) gave best disease control along with yield of cowpeas over leaf mold +20% neem cake formulation of the antagonist, talc base formulation and seed treatment with carbendazim at 2g/kg of seed. Seed priming with mycelial mat of the antagonist improved seed germination vigour of the cowpea plants, basal girth, number of branches & pod number, pod vigour of the bio primed seeds over other treatme...
P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi
Botanicals for control of anthracnose of bell peppers
...icus when compared to chemical fungicide Bavistin and also 13% reduction in disease incidence is observed when compared to untreated control. From the results it is evident that C. aromaticus were effective in not only controlling the disease but also increase the height and weight of the plants and hence are highly recommended for plant disease control.
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S.K. Sahoo
Incidence and management of mustard aphid (Lipaphis ery-simi Kaltenbach) in West Bengal
.... Among the different chemical insecticides evaluated for their bio-efficacy against L. erysimi, Dimethoate 30EC and Oxydemeton-methyl 25EC were proved to be more effective. The plots treated with Dimethoate and Oxydemeton-methyl recorded minimum aphid infestation in most of the observations, there by produced more yield ranging from 1151.6 to 1310.3 kg seed/ha. Incremental cost benefit ratio indicated that most favourable return was obtained under Dimethoate ...
Goutam Samui and Shantanu Jha
Branch gall of mango (Oligotrophus mangiferae Keiffer) its bioecology and management
... biology, population dynamics and management of this pest. Branch gall is a univoltine species. Emergence of adults from gall could be found from 2nd week of February, which continued up to 2nd week of March. Incubation, larval and pupal period lasted for 3-5 days, 315 days and 30-45 days, respectively. Eggs were laid singly throughout the young vegetative shoots by puncturing the tissue. A full grown larva was yellow in colour with a clear dark brown constric...
Madhuban Gopal, Rajesh Kumar and Arunava Goswami
Nano-pesticides - A recent approach for pest control
...nate use of synthetic chemical pesticides has led to several environmental problems such as resistance and environment pollution. Nanotechnology visualized as a rapidly evolving field has potential to revolutionize agriculture and food systems. We utilized nano-technological interventions for developing nanopesticides with potent fungicidal and miticidal properties. Nanosulphur and nanohexaconazole were prepared using encapsulation technique. The Dyna
S. Patra, V.W. Dhote, SK F. Alam, B.C. Das, M.L. Chatterjee and A. Samanta
Population dynamics of major insect pests and their natural enemies on cabbage under new alluvial zone of West Bengal
...justify;">Population dynamics of major insect pests and their natural enemies on cabbage were studied at Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, West Bengal(India) during rabi season of 2011-12 and 2012-13. Experiment was laid out in Randomized Block Design with three replications. Cabbage (cv Rareball) seedlings were transplanted in the plot of 9 m2 area with 45cm x 45cm spacing. Observation was recorded at weekly interval from randomly selected...
Lakshman Chandra Patel and Chandan Kumar Mondal
Management of causal agents of chilli leaf curl complex through bio-friendly approaches
...r study including two chemicals i.e. profenophos and diafenthiuron, three ecofriendly approaches i.e. garlic extract, spirit and alternate use of garlic with spirit, one predator insect Chrysoperla carnea and untreated control. The lowest population of thrips, mites and white fly was recorded with application of diafenthiuron at 15 days interval from seedling to early fruiting stage. Among the ecofriendly options, alternate use of garlic with spirit performed ...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...esearch areas were biochemical mechanism of resistance against phytonematodes and diagnostics of economically important plant parasitic nematodes. Specific isozymes have been identified for differentiating the important root knot nematodes species of India belonging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversi...
Anjan Bhattacharyya, Suhrid Ranjan Barik & Pritam Ganguly
New pesticide molecules, formulation technology and uses: Present status and future challenges
...dergo photo-degradation, microbial degradation as well as chemical degradation leaving very less amount of residues in the environment. Conventional pesticide formulations like WP, EC etc are endangering human health and polluting environment. Newly developed modern formulations like water emulsifiable gel, floating granules, drift less dust, macro and micro encapsulated suspension, hollow...
Amitava Konar and N. Johnson Singh
Occurrence of aphids on various potato germplasms in eastern gangetic plains of West Bengal
...ttern and population dynamics of aphids on various potato germplasms. The common potato germplasms viz Kufri Chandramukhi, Kufri Jyoti, Kufri Jawhar along with three processing varieties like Kufri Chipsona-1, Kufri Chipsona-2 and Atlantic were screened against aphids. Potato seed tuber was planted by end of November following all standard agronomic practices except insecticide application. The aphid species viz. Myzus persi...
Anil Sehajpal, Saroj Arora and Parminder Kaur
Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...wed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude of inh...
Dhananjoy Mandal
Eco-friendly management of mealybug and wilt in pineapple
...agement of mealy bug, Dysmicoccus brevipes Cockerell in pineapple, the treatments were: T1 [Farmers’ practice: phorate 10 G @ 20 kgha-1 during planting + monocrotophos 36% EC @ 0.03% at 100 DAP + endosulfan 35% EC @ 0.02% during 150-180 DAP]; T2 [Treating planting materials (basal portion) with monocrotophos 36% EC @ 0.02% + phorate 10 G @ 15 kg ha-1at 100 DAP + Neem oil 1500 ppm spray @ 2.5 m/L-1 at 150 DAP]; T3 [Treating planting materials (basal porti...
Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta
Developing site-specific appropriate precision agriculture
...and energy balance, economics ~ each with several variables. We are also providing ground and prepare to provide PA –specific data to the farmers. By Return per Rupee invested Rice-Potato-Pumpkin1, Cucumber-Cabbage-Basella alba, and Groundnut–Brinjal+Brinjal sequences were suitable for resource-rich growers, whereas Okra-Chilli+Chilli2 , and Black gram–Parwal+Parwal sequences were suitable for resource-poor growers. Overall, Groundnut–B...
T. P. Ghosh, D. Mandal, S. Laha and M. K. Dasgupta
Dynamics and severity model in managing fungal diseases
...ornamentals. Monthly dynamics was determined for occurrence, intensity and severity of these diseases. The diseases (referred as pathogens) that were the highest and fastest during warm and wet (rainy) months were: Alternaria alternata on Polyanthes tuberosa; Alternaria alternata and Septoria chrysanthemella on Chrysanthemum indicum; Colletotrichum gloeosporioides and Alternaria sp. on Dracaena deremensis; Alternaria sp. and Cercospora hibisci-manihotis on Hib...
Shekharappa
Biological control of earhead caterpillar, Helicoverpea armigera Hubner in sorghum
...PV) and compared with chemical control with Melathian 5% @ 30 kg.ha-1 for the management of ear head caterpillar (H. armigera) in sorghum ecosystem of University of Agricultural Sciences, Dharwad. The mycoinsecticide N. rileyi was highly infective at both the dosages 1x108 conidia per litre & 2x108 conidia per litre against H. armigera as compared to Ha NPV 250 LE per hectare and chemical control in mitigating the pest p...
S. Choudhury and S. Pal
Population dynamics of mustard aphid on different Brassica cultivars under terai agro-ecological conditions of West Bengal
...study the population dynamics of mustard aphid on seven popular rapeseed-mustard cultivars. The incidence of aphid commenced from 52nd to 2nd standard week with a very low population levels on all the cultivars. The aphid (Lipaphis erysimi) population attained peak level from 7th to 9th standard week. The correlation coefficients between aphid population and different abiotic factors revealed contradictory results. Except for a few instances the weather parame...
N. Mandi and A. K. Senapati
Integration of chemical botanical and microbial insecticides for control of thrips, Scirtothrips dorsalis Hood infesting chilli
...em pesticide (54.2%) and microbial pesticide BT (43.43%) were found moderately effective. However, two sprays of acetamiprid and thiamethoxam followed by two sprays of neem pesticide and BT proved to be effective for management of thrips and it gave highest marketable yield, higher cost benefit ratio and percent reduction in thrips’ population.
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Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ted to compare the biochemical and histopathological changes in response to pathogenic field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...xt-align: justify;">Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with characteristic organ involvement and autoantibodies production. The pathogenicity and etiology of the disease has yet to be elucidated. It is presently accepted that environmental factors trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a number of autoimmune d...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...isplays a variety of antimicrobial, antioxidant and immunomodulatory as well as a number of other biological functions. Its potential can be exploited in several food applications. Keeping in view the increased demand of natural antimicrobial agents to control prevalence of food borne pathogens in final packaged food products as well as for food preservation/ biocontrol, this study was designed to isolate lactoferrin from mi...
Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...udy was conducted on the microbial flora isolated from gills and intestine tissues of commercially important finfish species: saddled seabream (Oblada melanura), gilthead sea bream (Sparus aurata), Bogue (Boops boops), horse mackerel (Trachurus mediterraneus), red mullet (Mullus barbatus), brushtooth lizardfish (Saurida undosquamis), grey mullet (Mugil cephalus), threadfin bream, (Nemipterus randalli), wh...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...ceedingly conserved glutamic acid to glutamine at site which was involved in coding of ASF1 like histone chaperone. Further presumption based on structural and functional analysis of mutated protein was anticipated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
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Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...administered to pregnant mice. LD50 of Cr VI, was calculated as 88mg/kg of B.W in pregnant mice. Among 05 groups of pregnant females (10 females in each group), three were treated with 50%, 25% and 12.5% of the LD50 of Cr VI, respectively, with one control and one vehicle control group. Fetuses were recovered on 18th day of gestation and fixed for morphometric and morphological studies. Exper...
P.P. Ghosh*, C. Ghosh, B. Mahato, A. Chakraborty, S.K. Bhattacharya and M.K. Bhattacharjya
...s. Thus, for the present micro-level situation vascular bacterial wilt malady can be addressed by prophylactic application of lime at 500 kg/ha and bleaching powder 20 kg/ha in the soil four weeks before transplanting followed by seed dressing with 20g bioinoculant formulation per kg seed, pre-transplanting seedling root treatment with 20g bioinoculant formulation per litre and spot soil application of 7 days old mixture of bioinoculant formulation and well ro...
1Sitesh Chatterjee, 1Chirasree Gangopadhyay and 2Santosh Kumar Roy
...with spraying of same chemical provided the best result against white heads due to stem borer followed by chlorantraniliprole granule application in seedbed or the same granular application in the main-field along with spraying of chlorantraniliprole/flubendiamide in the main-field. The highest grain yield of rice was obtained from plot receiving granule application with spraying of chlorantraniliprole in transplanted field followed by chlorantraniliprole gran...
P.P. Ghosh*, A. Chakraborty, S.K. Bhattacharya, C. Ghosh, B. Mahato and M.K. Bhattacharjya
...e. Proven biological, chemical and botanical (vegetable oil) approaches of management were tested against traditional practice and replicated among 10 different household at 10 villages. Results showed that grain dressing with both neem and mustard oil under botanical approaches performed significantly superior over other approaches and traditional practice in terms reduction in percent infestation (92%), increase in germination percentage (36.04%), 88% increa...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...trains of bacteria at 5 µg/ml, 40 µg/ml and 80 µg/ml concentrations versus their counterparts of Lapachol and above mentioned metallic salts....
Gadeeyya G and Ratna Kumar P.K.
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Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
...raction methods. Phytochemical analysis of leaves was performed on powder and extracts of leaves using already reported methods. Mineral analysis was done using atomic absorption spectrophotometer. Hair growth promoting effects were examined using alopecia mouse model. Phytochemical analysis demonstrated the presence of higherquantities of carbohydrates, proteins and secondary metabolites ...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...uses (AIVs) H5N1 are endemic in Egypt and extensive vaccinations are being applied for chicken flocks, which have maternal derived antibodies (MDA) for these viruses. To understand the interference of maternal derived immunity impact of maternal derived immunity interference in vaccination regimen in poultry, a total of one hundred day old commercial broiler chicks were vaccinated with LaSota. A group of chicks (n=40) were immunized using baculovirus (H5 and N...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
... (LSD), which is an economically important disease of cattle and is caused by the LSD virus (LSDV). LSD is endemic in most countries of Africa and Middle East and can be transmitted either by mechanical as well as intrastadial and transstadial routes. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. In this study, we analysed the G-protein-coup...
Amir Ismail1, Muhammad Riaz1,2*, Saeed Akhtar1, Amjad Farooq3, Muhammad Arif Shahzad1 and Ahmad Mujtaba4
...permissible limit (0.02 µg/g) set by Codex Alimentarius Commission in 53% milk samples. In 44% milk samples the level of Cd was found to exceed the permissible limit (0.0026 µg/g) set by International Dairy Federation (1979). The mean levels of Ni, Cu and Co were found in the normal ranges. The data for estimated daily intake of heavy metals through milk showed that infants are most prone towards heavy metal toxi...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...nd GSTT. Comparative genomics and syntenic analysis provided new evidences for better understanding on gene fates post whole genome duplication (WGD) of common carp. The expression patterns of all GST genes were established in various tissues, including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution...
Erol Akkuzu1,*, Hidayet Güzel2 and Özkan Evcin1
...uence the population dynamics and severity of damage in a forest. This study investigated the effects of stand composition and site index of pine forests on Ips sexdentatus (Boerner) populations. Two main objectives of this study were a) to compare the effects of two different black pine stands of site index-I (high site productivity) and site index-III (low site productivity) and b) to compare the pure and mixed pine forests concerning the abundance an...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8, Naimat Ullah Malik1 and Abdul Razzaq9
...nd were screened through microscopy. Results yielded an overall prevalence of 11.11%, with significant difference (P<0.05) among different study areas. Prevalence was highest in district Bannu (11.66%), followed by district Lakki Marwat and Kohat with 10% in each district. Statistical analysis revealed significant difference (P<0.05) among children of different age groups with highest disease prevalence in children of less than 5 years of age (21.40%) an...
Rafi Ullah1, Sarzamin Khan1,*, Abdul Hafeez1, Asad Sultan1, Nazir Ahmad Khan2, Naila Chand1 and Naseer Ahmad1
..., indicating higher economic benefit. However, the gross return per bird and profit per kg meat were higher for diet D4 (P < 0.05). It was revealed that replacement of soyabean meal with silkworm meal did not affect broiler performance and carcass quality; therefore silkworm meal may effectively be used as a low-cost protein constituent in the broiler finisher ration.
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