In Vitro Nematicidal Activity of an Endemic Plant Pulicaria boissieri Hook. F. Against Root-Knot Nematodes Antinematodal Activity of Pulicaria boissieri
In Vitro Nematicidal Activity of an Endemic Plant Pulicaria boissieri Hook. F. Against Root-Knot Nematodes Antinematodal Activity of Pulicaria boissieri
Rauf Khan1, Shazia Mansuri1,2*, Muhammad Abid1, Muneeba Khan2, Anjum Perveen2, Afshan Rehman3 and Rubina Noureen1
ABSTRACT
In this current study, the in vitro nematicidal activity of Pulicaria boissieri against root-knot nematodes has been investigated. The results revealed that all the solvent extracts contained anti-nematodal properties; however, variations in mortality rates were observed. Specifically, the chloroform extract of the leaves of P. boissieri exhibited 84% inhibitory activity against nematodes after 72 hours of exposure. The results indicated that exposure of nematodes for 72 hours resulted in the maximum nematicidal activity compared to exposures of 24 and 48 hours. It was concluded that leaf and stem extracts in chloroform and methanol solvents exhibited the highest activity against nematodes. The sequence of nematicidal activity from high to low was as follows: chloroform > methanol > acetone > ethanol > water. Hence, Pulicaria boissieri demonstrates potential nematicidal activity with various organic solvents.
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