Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
Siddra Tayyab Akhtar, Anjum Nasim Sabri
ABSTRACT
Motility is a dynamic and remarkable element of bacterial physiology and
determines the ability of bacteria to form biofilms. There is a need to control
bacterial motility. Present study determined swimming, swarming, and twitching
motilities in response to different physiochemical factors. Twenty-two
morphologically different bacterial strains were collected from a drain of sewage
water located in the vicinity of Jinnah hospital Lahore, Pakistan. Six biofilm forming
bacterial strains were selected on the basis of their motility and biofilm forming
ability firstly by growing them on congo red agar medium and then by performing
ring test. By 16S rRNA sequencing, bacterial strains were identified as
Exiguobacterium aurantiacum, Bacillus subtilis, Bacillus megaterium, Bacillus
endophyticus, Pseudomonas fragi and Bacillus subtilis. The optimum temperature
and pH for swimming, swarming and twitching motilities was 37°C and pH 7. All the
bacterial strains showed reduction in diameter of zones of all motilities when the
concentration of NaCl increased in medium. Among chemical factors, tobramycin
was proven to have excellent efficiency to reduce bacterial motilites whereas
proteinase K enzyme showed weak antimotility effect against all tested bacterial
strains. From this study we proposed that instead of biofilm detachment by
expensive commercial detergents, we could change the physical environment of
sewage systems as well as flooding some specific chemicals, which disrupt
bacterial motilities and biofilm formation.
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