Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus
Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus
Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir
ABSTRACT
Background: Infectious bronchitis virus (IBV) is an acute contagious disease cause severe economic
losses in poultry production worldwide. Virus isolation is the golden standard for IBV diagnosis so the
aim of this study was comparing the different biological systems for isolation of infectious bronchitis
virus.
Methods: The Egyptian variant 2 IBV strain representative to the current circulating strain in Egypt
was isolated in specific pathogen free (SPF) embryonated chicken eggs ECE), chicken embryo kidney
cell (CEK) and in Vero cell line and was confirmed for IBV detection by Reverse Transcriptase Real
time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done
by generating a standard curve by real time PCR.
Results: The results revealed that the propagated virus in CEK showed the highest virus titer (5.952 x
106 and 3.245 x 106) than Vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x
105) after both 48 and 72 hours respectively.
Conclusion: The chicken embryo kidney cell (CEK) is the most successful and sensitive monolayer
cell culture host system for isolation of the Egyptian variant 2 strain of infectious bronchitis virus
(IBV)
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January 2018
Vol. 3, Iss. 1, Pages 1-77
