Therapeutic Effect of Red Ginger Leaf Ethyl Acetate Fraction Gel on Staphylococcus aureus-Infected Excision Wounds on Rat: A Preclinical Study
Therapeutic Effect of Red Ginger Leaf Ethyl Acetate Fraction Gel on Staphylococcus aureus-Infected Excision Wounds on Rat: A Preclinical Study
Bunga Rimta Barus1, Masfria Masfria2*, Aminah Dalimunthe3, Sovia Lenny4
ABSTRACT
A wound is an injury caused by a disruption in the body’s protective barriers resulting in damage to a specific area of the skin. Insufficient wound care may lead to further complications such as wound infections. Staphylococcus aureus is a pathogenic bacterium capable of causing wound infections. This study aimed to evaluate the effectiveness of gel formulations containing the ethyl acetate fraction obtained from red ginger leaves in facilitating the healing process of excision wounds. The study utilized 30 male Wistar rats, divided into six groups with five rats each, to ensure robust statistical analysis. The ethyl acetate fraction was extracted using a detailed maceration and fractionation process with 96% ethanol, followed by formulation into a gel. The gel formulations were evaluated for their antimicrobial properties and effectiveness in facilitating wound healing. The findings demonstrated that gel formulations containing 1%, 3%, and 5% concentrations of red ginger leaf extract exhibited significant antibacterial activity against Staphylococcus aureus, with the 5% concentration showing the largest zone of inhibition (11.20 ± 0.30 mm). Histological analysis revealed that the 5% gel concentration significantly enhanced epidermal thickness (52,407.77 ± 1.34 nm) and promoted angiogenesis (26.33 ± 0.31) in the wound area. These results indicate that the 5% ethyl acetate fraction gel is the most effective in promoting wound healing by enhancing both epidermal regeneration and angiogenesis.
Keywords | Antibacterial, Ethyl acetate, Fraction, Gel, Staphylococcus aureus, Wound
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