Internalization and Nuclear Localization of Porcine Growth Hormone in Porcine Hepatocytes
Internalization and Nuclear Localization of Porcine Growth Hormone in Porcine Hepatocytes
Pan Hong, Ruonan Li, Yumeng Li, Hainan Lan* and Xin Zheng*
ABSTRACT
The internalization and subcellular distribution of growth hormone (GH) from humans and rats have been extensively reported. However, to date, there are no descriptions of the cell behaviour of porcine GH in domestic animals. Thus, freshly isolated porcine hepatocytes were used as a somatic cell model to study the internalization and subcellular localization of porcine growth hormone (pGH), and the results showed that pGH could be internalized into porcine hepatocytes in a time-dependent manner. The results also showed that pGH could be translocated into cell nuclei in a time-dependent manner. In addition, the subcellular localization, according to immunoelectron microscopy, showed that pGH was localized in cytoplasmic organelles, e.g., the mitochondria and Golgi apparatus. The present study shows the first exploration of the behaviour of pGH in a somatic cell model of a domestic animal (pig), providing a basis for further studies on the physiological function of pGH based on its subcellular localization.
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February 2018
Vol. 50, Iss. 1, Pages 1-400
