Optimization of sporulation conditions of Bacillus sublilis as potential probiotics strain
Optimization of sporulation conditions of Bacillus sublilis as potential probiotics strain
MUHAMMAD SHRAFAT ALI1, UZMA HAMEED2* & IKRAM-UL-HAQ3
ABSTRACT
Probiotics are used as a substitute for antibiotic growth promoters in the chick industry. In the present study, conditions for the sporulation of Bacillus subtilis, a probiotic strain, are optimized. Overnight grown inoculum (1%) was used for the cultivation of B. subtilis in NB medium followed by 24-48 hours incubation in a shaking incubator at 30°C. Spore staining and microscopy were performed at different time intervals to find out the sporulation status and time required for the completion of sporulation. However, sporulation of the Bacillus strain completed after 22 hours and the total viable count (TVC) was found to be 8.3 x 1023 CFU/ml (OD 1.872). Similarly, optimization of temperature (30°C) and pH (7) was carried out that reduced the sporulation period up to 18 hours. The total viable count was 6.4 x 1012 CFU/ml. The acid tolerance of the B. subtilis was also determined at pH 2 and TVC after the acid treatment was 6.4 x 1012 CFU/ml. It depicts the ability of bacteria to withstand the acidic conditions.
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