Cholecalciferol and Formic Acid Synergistically Enhance Digestive Enzymes Activity and Bone Mineralization in Ctenopharyngodon idella
Cholecalciferol and Formic Acid Synergistically Enhance Digestive Enzymes Activity and Bone Mineralization in Ctenopharyngodon idella
Laiba Shafique1*, Mahroze Fatima2, Syed Zakir Hussain Shah3, Muhammad Afzal4, Huma Naz5, Saif ur Rehman1 and Qingyou Liu1*
ABSTRACT
This study was planned to determine intestinal digestive enzymes activity and bone mineralization of Ctenopharyngodon idella. Four type food were prepared by using formic acid (%) and vitamin D3 (IU/Kg) in experiment viz, FD1, FD2, FD3 and FD4, respectively. Fish fed with formic acid showed considerable increase in calcium, magnesium potassium, however vitamin D3considerably increase iron and manganese contents (µg/g) in the bones of C. idella. Amylase and protease activity was maximum in fish intestine fed with formic acid followed by fornic acid × vitamin D3, vitamin D3 = control diet. Activity of lipase enzyme was reduced by formic acid supplementation as well as vitamin D3. The observed formic acid and vitamin D3 (interactions) were synergistically bone mineralization of phosphorus, sodium, copper and also showed positive result for the activity of amylase, protease and lipase. Finally, formic acid and vitamin D3improves bone mineralization and digestive enzymes activity in the C. idella.
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