Using Bee Bread Extract as Natural Source of Energy, Antibiotics and Antioxidants Instead of Synthetic Sources During Freezing Steps of Ram Semen Extender
Using Bee Bread Extract as Natural Source of Energy, Antibiotics and Antioxidants Instead of Synthetic Sources During Freezing Steps of Ram Semen Extender
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
ABSTRACT
This study was conducted to assess the probabilities of using the ideal level of bee bear extract (BBE) up to 0.5, 1.0, 1.5, and 2.0 % for ram semen extender during freezing steps. Semen was collected by an artificial vagina from five adult Rahmani rams twice-weekly for five weeks. Semen was pooled, split into five replicates, and prepared to be frozen. In 1st replicate, semen was extended in Tris egg yolk (TEY) is contained synthetic sources of energy (fructose), antibiotics (penicillin and streptomycin), and antioxidants (vitamin E), which served as control extender (E0). However, 2nd, 3rd, 4thand 5th replicates were serviced as trial extenders as E1, E2, E3, and E4, which supplied 0.50, 1.00, 1.50, and 2.00 % of BBE as natural sources of energy, antibiotic and antioxidant, respectively. Results indicated that adding BBE to E1, E2, E3, and E4 extenders has increased (P<0.05) in all sperm characteristics and biochemical activity of extender as compared to E0 extender through semen extender preservation. The best level of BBE is marked when added up to 1.5% in the E3 extender which improves all characteristics compared to E0 and other BBE extenders. Also, E3 indicated post-thawing sperm parameters as motility, live, normal, and integrity acrosome up to 47.50, 65.10, 65.66, and 69.67%, but it was 37.22, 49.78, 55.89, and 59.22% relative to E0 extender, respectively. Furthermore, ATP, BC, and LPO levels post-thawing were 0.35µM, 255.40 CFU/ml, and 2.61 µM with E3 extender however, 0.19 µM, 276.00 CFU/ml, and 2.89 µM with E0 extender, respectively. Then, all of their previously enhanced post- thawing semen characteristics reflected higher positive values on its fertility (46.15 and 53.85%) and productive performance (53.85 and 69.23%) rates in E0 and E3, respectively. In conclusion, these results suggest that a ram semen extender adding 1.5% of BBE was optimum to obtain improved semen preservation.
Keywords | Rams, Semen extender, Bee bread extract, Sperm function, Fertility.
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