Isolation and Identification of Turkey pox virus in Turkey Flocks in Egypt
Isolation and Identification of Turkey pox virus in Turkey Flocks in Egypt
Safaa M. Mohammed*, Shahira A. Abdelwahab**,Fetaih,H⃰ ⃰ ,Neveen,R⃰ ,Abdel satar ,A⃰ and Mohamed S. Elshahidy**
ABSTRACT
In this study, turkey pox virus (TKPV) was recorded in 6 commercial turkey flocks aged 48-65days old. The disease was examined clinically ,histopathologically and immunohistochemically. Turkey pox virus was isolated on CAM of embryonated chicken eggs and identified by agar gel precipitation test and polymerase chain reaction .Two main types of TKPV infection were observed in examined flocks ,cutaneous and diphtheritic forms. Morbidity rate ranged from 5-15%with no mortalities .75% of examined serum reacted positive to TKPV .All TKPV isolates were amplified using specific P4b primers and visualized by gel electrophoresis at 578bp.
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