Submit or Track your Manuscript LOG-IN

Effect of Dietary L-Carnitine Supplementation on Growth, Serum Biochemical Indices, Oxidative and Growth-Related Gene Expressions in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Effect of Dietary L-Carnitine Supplementation on Growth, Serum Biochemical Indices, Oxidative and Growth-Related Gene Expressions in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Xing Lu1, Mahmoud A.O. Dawood2, Fan Wu1, Hua Wen1, Wei Liu1, Juan Tian1, Ming Jiang1, Li-Juan Yu1, Xiang Li3, Ning Xu3 and Hong-Wei Liang1*

1Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
2Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, 33516, Egypt.
3Anhui XIjia Agricultural Development Co. Ltd, Bengbu 233700, China
 
* Corresponding author: lianghw@yfi.ac.cn

ABSTRACT

This study was conducted to investigate the effects of dietary L-carnitine supplementation in Chinese soft-shelled turtle, Pelodiscus sinensis. Four experimental diets were formulated with the addition of L-carnitine at the levels of 0 (control), 100, 200 and 400 mg kg-1. Each diet was fed to triplicate groups of turtles with initial average weight (394.4 ± 3.8 g) for 8 weeks. Our results showed that the weight gain rate (WGR), specific growth rate (SGR) and feed efficiency (FE) of Chinese soft-shelled turtles increased linearly with increasing dietary L-carnitine levels from 0 to 200 mg kg-1, but declined slightly with the L-carnitine supplementation at 400 mg kg-1. Muscle protein content was significantly increased when turtles were fed diets with 200 or 400 mg kg-1 L-carnitine (P < 0.05). The serum biochemical indices analysis revealed that dietary L-carnitine at 400 mg kg-1 had markedly reduced triglyceride (TG) concentration (P < 0.05). The elevated expression of hepatic igf1 gene at the transcriptional level was positively correlated with dietary L-carnitine levels on the growth performance of Chinese soft-shelled turtle. Furthermore, dietary L-carnitine supplementation significantly up-regulated the mRNA levels of superoxide dismutase 1 (sod1) and lipoprotein lipase (lpl) genes in the liver of Chinese soft-shelled turtle (P < 0.05). All the above results indicated that appropriate dietary L-carnitine supplementation could improve growth performance, increase muscle protein content, reduce serum TG concentration, and induce the antioxidant- and lipometabolism-associated genes expression in Chinese soft-shelled turtle.

 

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe