Bioremedy of Cotton Aphid (Aphis gossypii Glov.) (Hemiptera: Aphididae) by the Application of Different Fractions of Entomopathogenic Bacteria (Xenorhabdus Spp.)
Bioremedy of Cotton Aphid (Aphis gossypii Glov.) (Hemiptera: Aphididae) by the Application of Different Fractions of Entomopathogenic Bacteria (Xenorhabdus Spp.)
Erum Yawar Iqbal1*, Ashfaque Ahmed Nahiyoon1, Shahnaz Dawar2 and Shahina Fayyaz1
ABSTRACT
Molecularly identified indigenous entomopathogenic bacteria (EPB) Xenorhabdus have been used to access their efficiency against cotton Aphid Aphis gossypii (Glov.). An efficient formulation of different parts of bacterial culture such as, cell-free supernatant, crude cell extract, bacterial culture and methanol extract in in vitro and green house condition have been exploited. The results have shown that different formulants of EPB produced significant effects on Aphid mortality, change in population density, population reduction and fecundity. Cell-free supernatant of new EPB species Xenorhabdus steinernematis n.sp. strain PAK. CB10 (KU097324) and the other X. indica PAK. S.B.56 (MF521953) resulted in both the highest mortality rate [(94.33±2.05, 100.00±0.00)%] @ 300µl/10ml at 30ºC and the lowest fecundity [(65.00±3.00) eggs/gravid female] in green house condition after 5 days of treatment. Crude cell extract of all bacterialfractions were found to be least effective as compared to cell-free supernatant, bacterial suspension and methanol extract suggesting that EPB has the potential to release its metabolites with insecticidal mode of actions in the surrounding culture media. New species proficiency was evaluated in comparison with other indigenous isolates and resulted in most efficient at 30ºC after 24h and proved to have good effectiveness as biological control agent and can be easily used instead of live bacteria in future formulations.
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June 2020
Vol. 52, Iss. 3, Pages 825-1224
