Fertility Rate, Fertility Period, and DOC Sex Ratio of Kokok Balenggek Chicken after Artificial Insemination
Fertility Rate, Fertility Period, and DOC Sex Ratio of Kokok Balenggek Chicken after Artificial Insemination
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
ABSTRACT
This study aims to evaluate the characteristics of fresh semen of the Kokok Balenggek chicken (KBC) as well as fertility, fertile period, and sex ratio as a result of artificial insemination with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and fertility were evaluated by inseminating spermatozoa in hens. Eggs were collected two days after insemination and observed for embryo development using the candling method. Observation of fresh semen of the KBC showed that macroscopically the semen of the KBC was white, thick, and had a volume of 415 ± 218.08 µl. While the results of the microscopic evaluation showed that the semen quality of the KBC was a positive mean mass movement of 2 (++), spermatozoa motility was 79.71 ± 2.91 %, spermatozoa concentration was 2432.34 ± 668.08×106, spermatozoa viability was 93.06 ± 5.61 % and spermatozoa abnormality was 15.67 ±7.07 %. Insemination using a concentration of 150×106 spermatozoa significantly (P<0.05) increased fertility (50.87±4.92 %) and fertile period (6.05±4.75 days) and did not affect the sex ratio. Combination research on spermatozoa concentrations and insemination time intervals needs to be carried out to optimize fertility and the fertile period of spermatozoa in KBC.
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