Effects of Green Tea Polyphenols, Vitamin E, and Ocimum gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality
Effects of Green Tea Polyphenols, Vitamin E, and Ocimum gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
ABSTRACT
During chilled storage oxidative stress is the primary cause for the reduction in the longevity of canine sperm. To prolong the chilled canine sperm quality, the study investigated the effects of the adding antioxidants to semen extender on canine sperm during 12 days of chilling storage. Twenty ejaculates from five American Bully dogs were used. The sperm quality was determined by system automatic sperm analyser for sperm motility parameters, and by confocal laser scanning microscope for sperm membrane integrity parameters. Sperm lipid peroxidation was also analysed through malondialdehyde production. The study found that there was a gradual decrease in all sperm quality indicators of all the treatments during 12 days of storage. In addition, the chilled sperm motility parameters among all the semen extenders were not noticeably different at each time period of evaluation (P>0.05). Although sperm lipid peroxidation index in the Ocimum gratissimum essential oils treatment was higher than those in the treatment of vitamin E and green tea polyphenols, the percentage of the high mitochondrial membrane potential, the intact plasma membrane and acrosome membrane indicators in the Ocimum gratissimum essential oils treatment were higher than those in the rest treatments and had a substantial difference when compared to the control treatment (P<0.05). In concluding, semen extender supplemented with Ocimum gratissimum essential oils is appropriate for improving the chilled canine sperm for the duration of 12 days in storage.
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