Sequence Diversity of Interferon Beta-1 Gene in Dromedary Camel Breeds of Pakistan
Sequence Diversity of Interferon Beta-1 Gene in Dromedary Camel Breeds of Pakistan
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
ABSTRACT
With the imminent danger of global climate change threatening the Earth’s biodiversity, the camel proved to be a unique animal with physiological features. These attributes of the camel instigate us to explore its genetic architecture. Pakistan boasts a camel population of about 20 different breeds and a total of one million head. However, till date, scarce genetic data is available on Pakistani camel’s genomics. In efforts to explore the genetics of the camel, we chose to study the Inteferon beta 1 (IFNβ1) gene in in Mareecha camel and non-descript camel breeds in Pakistan. We amplified the IFNβ1 gene through PCR, followed by sequencing. All sequences were aligned and edited through Codon Code aligner and finally a 364 bp portion was selected for further analysis. Dual peaks/heterozygous conditions were identified at positions c.36 (A/C), c.87 and c.225 (Y, T/C). The phylogenetic tree was constructed through MEGA 6.1 software package using the Neighbor Joining method with a bootstrap value of 1000. Finally, the dromedary camel sequences from Pakistan were compared with corresponding genetic sequences of other animal species available on NCBI including European bison, Indian bison, Tibetan antelope, wild yak, domestic goat, sheep, water buffalo and cattle for biological positioning of dromedary camel. The results reconfirmed the classical biological classification.
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June 2021
Vol. 53, Iss. 3, Pages 801-1200
