Statistical Optimization of Alkaline Protease Production by Newly Isolated Bacillus Strain using Industrial Skin Waste as a Novel Substrate
Statistical Optimization of Alkaline Protease Production by Newly Isolated Bacillus Strain using Industrial Skin Waste as a Novel Substrate
Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3
ABSTRACT
Abstract | Protease is one among other many enzymes which possess the qualities of the vitally important hydrolytic enzymes. The present experimental work of the protease production was conducted by using skin waste from leather industry as a substrate. Different sources of nitrogen and carbon were screened in order to enhance the production of protease by a locally isolated Bacillus sp. In order to ensure the best results Response surface methodology (RSM) was employed to optimize five different independent variables including substrate, Molasses, temperature, pH and incubation period. Maximum protease activity was observed by using molasses (540.62 ± 26.77 IU/ml) as carbon source and NaNO3 (606.95 ± 7.61 IU/ml) as nitrogen source. The optimum conditions predicted by RSM were substrate 2%, molasses 1.5%, temperature 35 °C, pH 9.0 and induction period of 96 hour. Almost 2.9 fold increase in protease production was observed after optimization at these optimal conditions. All these results indicated that skin waste from leather industry can be exploited as a potential substrate for alkaline protease production at optimized conditions by Bacillus sp.
Novelty Statement | In present research work industrial skin waste obtained from the local leather industry was exploited as substrate for alkaline protease production that make the process economically feasible and environment friendly by utilizing the industry waste.
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December 2021
Vol. 36, Iss. 2, pp. 125-252
