The purpose of this study was to determine effect of partial or complete replacement of corn grains by dried sugar beet pulp on rumen parameters. The yellow corn was substituted by dried sugar beet pulp at 25 (D1), 50 (D2), 75 (D3), and 100% (D4). Results of chemical composition analyses indicated that DM, OM, ash and CP contents did not differ among treatments. Crude fiber content increased linearly with increased dried sugar beet pulp percentage in rations, with significant differences in D2, D3 and D4. Content of NDF was significantly higher in all experimental groups when compared with control group, whereas non-fibrous carbohydrates content decreased in all groups as the effect of corn grains replacement by dried sugar beet pulp. Complete substitution of yellow corn by sugar dried beet pulp in D4 significantly increased number of bacteria. D3 and D4 diets resulted in higher (P<0.001) concentration of Holotrichs. The ratio of acetate to propionate increased linearly with inclusion of dried sugar beet in the diet, however statistically significant differences were found only in D4. On basis of presented data, it could be concluded that yellow corn could be partially of fully substituted by dried sugar beet pulp without negative impact on rumen parameters including dry matter digestibility.