The Quality of KUB Rooster Sperm During Cryopreservation in Extender with Genistein and Glutathione Supplementation
The Quality of KUB Rooster Sperm During Cryopreservation in Extender with Genistein and Glutathione Supplementation
Makruf Arif1, Asmarani Kusumawati2*, Kurniawan Dwi Prihantoko3, Agustina Dwi Wijayanti4, Sri Gustari2
ABSTRACT
The increase in lipid peroxidation during rooster semen cryopreservation reduces sperm functioning and creates reproductive issues. Antioxidants are crucial in semen cryopreservation to inhibit lipid peroxidase, thereby preserving sperm quality. This study aimed to investigate the effects of genistein and glutathione supplementation on the quality of cryopreserved sperm. Semen was collected from six KUB roosters and then diluted with the LREY extender using five different treatments: LREY without antioxidant as control (LREY0), LREY supplemented with genistein at 5 µM (LREY5), genistein at 10 µM (LREY10), glutathione at 0.2 mM (LREY2), and glutathione at 0.4 mM (LREY4). The semen was evaluated after dilution, equilibration, and thawing. The evaluation criteria included sperm motility, recovery rate, viability, membrane integrity, and DNA fragmentation. In the post-dilution and post-equilibration phases, there was no significant effect on motility, viability, and membrane integrity (P > 0.05). However, supplementing genistein and glutathione improved the post-thawing results significantly (P < 0.05). The addition of 10 µM genistein and 0.02 mM glutathione to KUB rooster semen post-thawing improved motility (43.00±1.41% and 41.75±1.70%), viability (54.75±1.70% and 53.75±1.50%), membrane integrity (57.50±2.64% and 57.25±2.21%), recovery rate (51.19±1.68% and 49.69±2.03%), and DNA fragmentation (3.25±0.50% and 3.25±0.95%). This study concluded that genistein at 10 µM and glutathione at 0.2 mM may preserve the quality of KUB rooster semen.
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