Studying the Anti-Diabetic Effect of Gamma-Irradiated Pumpkin Seeds
Studying the Anti-Diabetic Effect of Gamma-Irradiated Pumpkin Seeds
A.M. Abdul Azeem, Ashraf M. Mounir and Amr N. El-Shahat*
ABSTRACT
Natural antioxidants from plant materials have been consumed to replace synthetic ones as supportive therapy in the treatment of diabetes. This research was aimed to explore the influence of gamma (γ)-irradiation on the total phenolic compounds and total flavonoid contents of dried pumpkin seeds as well to determine the hypoglycemic influence of γ-irradiated pumpkin seeds dried powder (GPSDP) on diabetic rats. In this work, the level of total phenolic and total flavonoid contents of raw dried pumpkin seeds was significantly increase under the effect of γ-irradiation (10 kGy). The results showed that glucose and testicular malondialdehyde serum level and the activity of xanthine oxidase were elevated (P < 0.05) with obvious reduction in the concentration of insulin, total thyroid hormones triiodothyronin and thyroxine, leutinizing hormone, testosterone and testicular antioxidant parameters (glutathione content , the activity of xanthine dehydrogenase, superoxide dismutase and catalase) in the group of alloxan (150 mg/kg B.WT)-administrated rats as compared to control group. Treatment of diabetic rats with GPSDP (1 g/kg B.Wt/day/ 8 weeks) resulted in significant reduction of glucose level and lipid-peroxidation with amelioration of hormonal disturbance and enhancement of testicular antioxidant status with relative to alloxan-induced diabetic group. Therefore, this study concluded that γ-irradiation can be used as useful technique to improve the antioxidant activity of PSDP without loss in its quality characteristics. Also, the results suggested the effectiveness of GPSDP as a natural antioxidant that can reduce diabetic disorders.
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