In-Vitro Propagation of Neurada procumbensl L (Chipri Booti): An Endangered Medicinal Plant from Cholistan Desert
In-Vitro Propagation of Neurada procumbensl L (Chipri Booti): An Endangered Medicinal Plant from Cholistan Desert
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
ABSTRACT
Present investigations were carried out with a view to optimize an in-vitro culture protocol for propagation of an endangered medicinal plant Neurada procumbens L. from Cholistan desert in Pakistan. Nodal segments from healthy grown plants were used as explants and cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots per shoot at an average root length of 3.86 cm. After acclimatization and transplantation, 90% of the In- vitro derived plants were found healthy in ex vivo conditions.
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March 2018
Vol. 31, Iss. 1, Pages 1-105
