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Effect of temperature on emergence of Steinernema feltiae from infected Galleria mellonella cadavers under moist and dry conditions

Effect of temperature on emergence of Steinernema feltiae from infected Galleria mellonella cadavers under moist and dry conditions

A. M. Rahoo1,*, †, T. Mukhtar2, S. I. Abro3, S. R. Gowen1 and B. A. Bughio4

1School of Agriculture, Policy and Development, University of Reading, Reading RG6 6AR, UK
* Present Address: Wheat Research Institute, Sakrand, Sindh, Pakistan
2Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
3Department of Soil Science, Sindh Agriculture University, Tandojam, Pakistan
4Department of Zoology, University of Sindh, Jamshoro, Pakistan.
†Corresponding author: [email protected]

ABSTRACT

The management of insect pests is mainly relied on synthetic insecticides which are inimical to humans, livestock
and environment. To dispense with the pernicious consequences of chemicals, use of entomopathogenic nematodes
(EPNs) is among the viable alternatives. The successful management by EPNs is dependent on their establishment in
soils, infectivity, persistence and pathogenicity for longer periods. In the present study, emergence of Steinernema
feltiae infective juveniles (IJs) from infected Galleria mellonella cadavers was monitored under moist and dry
conditions at 5 and 10oC. Greater numbers of IJs of S. feltiae recovered from G. mellonella cadavers kept at 10oC
than from those kept at 5oC. Likewise, significantly greater number of infective juveniles emerged in moist
conditions as compared to dry. The relationship between both the temperatures and wet and dry conditions was
highly significant (P<0.001).

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Pakistan Journal of Nematology

June

Pakistan Journal of Nematology, Vol. 42, Iss. 1, Pages 1-87

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