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Acetylcholinesterase Inhibitory Activity of Venom of Hottentota tamulus (Fabricius) and Androctonus finitimus (Pocock) (Scorpiones: Buthidae) in Musca domestica

Acetylcholinesterase Inhibitory Activity of Venom of Hottentota tamulus (Fabricius) and Androctonus finitimus (Pocock) (Scorpiones: Buthidae) in Musca domestica

Naila Riaz1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1, Ali Hassan2, Shaukat Ali2, Shafaat Yar Khan1

1Department of Zoology, University of Sargodha, Sargodha, Pakistan
2Department of Zoology, Government College University, Lahore, Pakistan

Corresponding author: Hafiz Muhammad Tahir, hafiztahirpk1@yahoo.com

 

ABSTRACT

In the present study, different venom fractions were isolated from the crude venom of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) by HPLC. The toxicity and acetylcholinesterase activity of selected venom fractions were determined using of Musca domestica adults. Only one fraction (out of 7) from H. tamulus venom and one fraction (out of 5) of A. finitimus venom were selected on the basis of their maximum yield. The approximate molecular weights of these selected venom fractions were determined by SDS Gel electrophoresis. The molecular weight of venom fractions of H. tamulus and A. finitimus used in the study were 36kDa and54kDa, respectively. The doses of venom fractions used during the study ranged from 10-30µg/ml. Both venom fractions significantly inhibited the activity of AChE. However, 30µg/ml dose of both scorpion venom caused 100% mortality in adult M. domestica. . It was concluded that the venom of scorpions contains peptides fractions that may be used as potential bio-pesticides.

 

 

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Punjab University Journal of Zoology

December

Vol. 35, Iss. 2, pp. 167-268

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