Influence of Honey on Sperm Traits in KUB Rooster Concerning Cold Storage
Influence of Honey on Sperm Traits in KUB Rooster Concerning Cold Storage
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
ABSTRACT
Damage due to oxidative stress during semen preservation can cause a decrease in motility, viability, and damage to plasma membranes that can reduce fertility rates. The use of antioxidants in semen preservation is very important to suppress lipid peroxidation so it can maintain the quality of the sperm. This study aimed to determine additional honey to the quality of KUB rooster sperm during cold storage. The semen with superior quality was diluted into four different treatments, lactate ringer egg yolk without honey as control (LREY0), LREY with 0.2% honey (LREY2), LREY with 0.4% honey (LREY4), and LREY with 0.6% honey (LREY6). Semen was stored at 5°C for 48 hours. The evaluation of semen quality includes an examination of spermatozoa motility using a microscope, the viability with the eosin-negrosin staining method, and membrane integrity using the hypoosmotic swelling test (HOST) at the fresh semen, 5th, 24th, and 48th hours post-cold storage. The results revealed that in the 5th hour, percentage of motility, viability, and membrane integrity were not significantly different. However, at the 24th and 48th-hour results revealed significant differences by adding honey (P < 0,05). The 0.4% honey supplementation was the best to maintain KUB rooster quality until the 48th hour with motility of up to 41.50±1.19%, the viability of up to 59.75±0.63%, and membrane integrity of up to 66.50±1.32%. It can be concluded from the current study that adding 0.4% honey can preserve the quality of KUB rooster sperm during cold storage.
Keywords | Antioxidant, Chilling storage, Honey, KUB rooster, Sperm membrane integrity
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February 2023
Vol. 11, Iss. 2, Pages 189-354
