Optimization of Cellulase Production by Chaetomium thermophilum in Submerged Fermentation using Wheat Straw as Substrate
Optimization of Cellulase Production by Chaetomium thermophilum in Submerged Fermentation using Wheat Straw as Substrate
Sabahat Anwar1, Faria Javaid2 and Qurat-ul-Ain2*
ABSTRACT
This study was carried out to produce thermally stable, economically viable and industrially important cellulase enzymes by using a thermophilic fungus i.e., Chaetomium thermophilum, which can efficiently produce cellulases extracellularly while growing on cellulosic substrates. Wheat straw, by product of wheat crop has been used as lignocellulosic substrate. To determine the optimum conditions for maximum production, experiment was carried out at various substrate concentrations, temperatures and pH levels. Pretreatment of wheat straw resulted in highly digestible cellulose that lead to the increased production of cellulases. Highest glucose (1.92±0.14 mg/ml), carboxymethyl cellulase (7.33±0.16 IU/ml/min) and filter paperase (10.61±0.15 IU/ml/min) activities observed in pretreated wheat straw, while untreated gave 1.89±0.19 mg/ml, 4.31±0.13 IU/ml/min and 7.88±0.18 IU/ml/min respectively. Seventy percent increase in CMC-ase and 35% increase in FP-ase activities were observed while using alkali pretreated wheat straw as compared to untreated straw. During incubation, pH 7 and 60°C temperature was found optimum for highest enzyme activities.
To share on other social networks, click on any share button. What are these?
