Hepatoprotective Potential of Mesembryanthemum forsskalii Fruits Extract Against Carbon Tetrachloride-Induced Liver Toxicity in Mice
Hepatoprotective Potential of Mesembryanthemum forsskalii Fruits Extract Against Carbon Tetrachloride-Induced Liver Toxicity in Mice
Usama Mahalel1, Barakat M. Alrashdi1, Ibrahim Abdel-Farid1, Sabry El-Naggar2, Mohamed Hassan3, Hassan Elgebaly1 and Diaa Massoud1,4*
ABSTRACT
The present study aimed to investigate the ameliorative effect of the methanolic extract of Mesembryanthemum forsskalii fruits extract against the hepatotoxicity of carbon tetrachloride (CCl4) in mice at two different doses (low; 100 mg/kg BW and high; 500 mg/kg BW). The obtained results showed that CCl4 injection significantly reduced the body weights of mice and increased the relative liver weight. Treatment with M. forsskalii extract after CCl4 injection restores the weights of mice to the normal range and reduced the relative weight of the liver. Mice that were injected with CCl4 exhibited elevation in the liver enzymes; aspartate aminotransferase (AST) and alanine aminotransferase (ALT), reduction in the cholesterol content, and altered the architecture of the hepatic cells. Animals treated with M. forsskalii extract at a low dose after CCl4 administration enhanced the liver functions as evidenced by histological examination and liver enzymes analysis. Immunohistochemical investigation showed that the administration of M. forsskalii extract at a low dose downregulated the expression pattern of P53 and upregulated the expression of Bcl-2 in liver tissue. In conclusion, our findings indicated that the methanolic extract of M. forsskalii at a low dose (100 mg/kg BW) attenuated the hepatotoxicity induced by CCl4 through upregulation of Bcl-2 expression and restoring liver enzymes to their normal range.
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