Transcriptome Analysis in the Fat Body of Two Silkworm (Bombyx mori) Strains with Different Susceptibility to Fenvalerate
Transcriptome Analysis in the Fat Body of Two Silkworm (Bombyx mori) Strains with Different Susceptibility to Fenvalerate
Guo-dong Zhao1,2*, He-ying Qian1,2, Yi-ling Zhang1,2, Gang Li1, 2, Jian Tang1, 2 and An-ying Xu1,2*
ABSTRACT
The silkworm, Bombyx mori, an economically important insect, is also used as a model insect for investigation. However, pesticide exposure often causes huge economic losses to the sericulture. The fat body is an important intermediate metabolic tissue, which is involved in detoxification in insects. In this study, high-throughput transcriptome sequencing was performed to investigate the gene expression differences between two silkworm strains with different susceptibility after exposure to fenvalerate. The results showed that a total of 2363 differentially expressed genes were detected in the Lan5 DGE library and 1611 were detected in the Mysore DGE library, respectively. The possible functions of all DEGs were determined using the gene ontology (GO) classification system. Pathway enrichment analysis was carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Furthermore, some selected differentially expressed genes (DEGs) were verified by qRT-PCR. Our results can facilitate the overall understanding of the mechanism of silkworm’s tolerance to pesticides and provide a new control strategy of Lepidoptera pests.
To share on other social networks, click on any share button. What are these?
