Molecular Characterization of Codling Moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) in Swat Valley Pakistan using Randomly Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction
Molecular Characterization of Codling Moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) in Swat Valley Pakistan using Randomly Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction
Hayat Zada1, Ahmad-Ur-Rahman Saljoqi1,*, Ijaz Ali2, Bashir Ahmad1, Abdul Waheed Khan2 and Shabeer Ahmad2
ABSTRACT
Codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) is the most important insect pest of apple production in Pakistan. Genomic DNA was extracted from 30 overwintering larvae of each population of Matta, Madyan and Kalam regions. Out of 30 tested primers, 21 amplified 157 polymorphic bands in three populations. The mean gene frequency (f), genetic distance (I) and Shannon’s information index (h) for three populations were 1.336, 0.304 and 0.437, respectively. Nei’s unbiased measures of genetic identity and genetic distance revealed that higher genetic distance was observed among the isolates from Kalam and Madyan (97.87 %) whereas low genetic distance (35.58%) was calculated for C. pomonella isolates from Matta and Madyan. These results revealed that molecular variations among the population of C. pomonella might be attributed to the climatic conditions, geographical location and pest control programs.
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August 2019
Vol. 51, Iss. 4, Pages 1203-1598
