Use of Suppression Subtractive Hybridization for Viral Diagnostics
Use of Suppression Subtractive Hybridization for Viral Diagnostics
Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
ABSTRACT
Efficient identification of viruses is paramount to not only define the cause of uncharacterized infections but also to estimate the dynamics of viral population in the infected hosts. Several recent methodologies and chemistries have been proposed for viral diagnostics with variable outcomes and successes. In this review, we discuss a novel approach to identify the least transcripts of viral genomes using suppression subtractive hybridization that can be adapted to unanimously identify viruses of diverse genetic backgrounds.
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April 2016
Vol. 3, Iss. 2, Pages 24-46
