Differentiation and anatomical changes in Callus cultures of Rauwolfia serpentine BENTH
Differentiation and anatomical changes in Callus cultures of Rauwolfia serpentine BENTH
Rehana Perveen, Mehmood Akram and Ihsan Ilahi
ABSTRACT
Callus induced in shoot pieces of Rauwaolfia serpentina
on Knop's medium supplemented with 1mg/1 of NAA
exhibited vigorous growth when transferred to MS medium
supplemented with 1 mg/1 both of Naphthalene acetic
(NAA) and Benzyl amino purine (BAP). The effect of
varying concentrations of NAA &BAP alone and in
combination with each other was studied on further
growth and differentiation of the callus. At NAA 1 mg/1,
BAP 0.5 mg/1 NAA 0.5 mg/1 roots developed at 0.5mg/1 of
BAP & NAA 0.5+1.0 mg/1 of BAP shoot buds also developed.
Callus pieces cultured on MS medium supplemented with
different combinations of NAA &BAP showed progressive
anatomical changes accompanying differentiation of roots
and shoot buds.
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September 1982
Vol. 32, Iss. 3
