Isolation, Identification, and Screening of Keratinase Producing Bacteria from Soil and Production Optimization Using Feather Waste as Substrate
Isolation, Identification, and Screening of Keratinase Producing Bacteria from Soil and Production Optimization Using Feather Waste as Substrate
Farha Deba1, Rubina Nelofer2 and Muhammad Irfan1*
ABSTRACT
In this investigation, seven distinct bacterial strains that can produce keratinase were isolated and identified from the abattoir region. A transparent, clear zone was evident in each isolate during the initial primary screening on skimmed milk agar plates. The secondary screening was then evaluated via submerged fermentation utilizing raw feathers as a substrate for keratinase production. On skimmed milk agar plates with streaked pure culture growth patterns, these seven strains gave a positive appearance of sizable dramatic zones around the growth patterns. These seven strains such as Bacillus velezensis FD1, Bacillus cereus FD2, Bacillus subtilis FD3, Bacillus altitudinis FD4, Bacillus licheniformis FD5, Bacillus flexus FD6 and Alcaligenes sp. FD7 were verified by the molecular identification investigation using 16S rRNA sequence technology respectively. Out of these seven isolates, the Bacillus cereus FD2 strain produced the most keratinase (298U/mL), and was selected for more research. The Bacillus cereus showed the highest keratinase activity at neutral pH 7.0, with 1% inoculum size, 1% substrate and after 72h of the incubation period. This current study revealed that these isolates have ability to be used in many biotechnologies prospective applications like in hydrolysis of keratin-containing substrate. Recently, it has been extremely beneficial and a major priority to use microorganisms for the enzymatic destruction of keratin waste material rather of various conventional procedures that are expensive and not ecologically friendly.
Novelty Statement | The study reports proteolytic/keratinolytic bacteria for the first time from abattoir region.
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June 2023
Vol.38, Iss. 1, Pages 01-135
