Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1
ABSTRACT
The present study was aimed to assess the optimum conditions to cultivate the yeast, Candida tropicalisSS1 for the single cell protein (SCP) production employing different fruit’s peels. The C. tropicalis SS1 was cultivated in media containing 2% pulverized peels of apples, mangoes, water melon and bagasse singly as well as in eleven different combinations. The media were inoculated with 1% (w/v) suspension of 24 h old yeast cultured in nutrient broth. The strain grew best in water melon (WM) at pH 7.0 and 37 °C under non agitation conditions. The proximate analysis revealed that the water melon peels showed the highest amount of crude protein (which was measured by micro Kjeldahl digestion system) followed by bagasse, mango, and apple peels with respective values of as 13.38%, 3.20%, 2.69% and 2.58%. Best growth of C. tropicalis SS1 was obtained in aqueous extracts of 2% WM peels and the yeast cells attained up to 33% protein content on dry weight basis. In 2% aqueous extract of WM, the yeast strain yielded maximum biomass up to 6g/L with corresponding absorbance(600 nm) of 3.49±0.05 after 48 h of incubation at 37 ºC, pH 7.0 and under non aerating conditions. These results indicate important biochemical attributes of the abundantly produced fruit wastes and their efficiency for economic production of C. tropicalis SS1 which could be used as SCP supplementing animal feed’s formulations.
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